Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells.

Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells. Research Abstract Details 

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Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells. Abstract Text:

Lihuan Cao,Rongfa Bu,Jennifer I Oakley,Sara E Kalla,Harry C Blair,

Estrogens have complex effects on the skeleton, including regulation of modeling and maintenance of bone mass, which vary with cell type and developmental stage. Osteoblasts are key regulators of skeletal matrix synthesis and degradation. However, whether osteocytes, osteoblasts or earlier progenitors mediate estrogen effects, and the importance of estrogen receptors (ERs) alpha and beta, remain unclear. To address estrogen response in human cells closely related to secretory osteoblasts, we studied MG63 cells with ERalpha or ERbeta reduced to low levels by stable transfection of antisense plasmids. Collagen and alkaline phosphatase expression increased with estrogen in wild-type and ERalpha-suppressed cells, but not in ERbeta-suppressed cells. Matrix secretion occurs as osteoblasts cease dividing, and, in keeping with this, cell proliferation was reduced by estrogen except in ERbeta-antisense cells. No effects of estrogen on wild type or ER-suppressed cells were seen in expression of BMP 2, the BMP antagonist noggin, or Indian hedgehog, products that regulate differentiation of osteoblasts. In contrast to expectations that estrogen would modulate bone degradation, RANKL, CSF-1, and osteoprotegerin did not respond measurably to estrogen, regardless of ER status. In keeping with this result, estrogen response was not observed in assays of osteoclast development from CD14 cells supported by wild-type or ER-silenced MG63 cells. Since estrogens are major regulators of bone degradation in vivo, estrogen effects on osteoclasts may depend on interaction with stimuli present in bone but absent in the model studied. cDNA hybridization showed that additional estrogen-binding proteins including ERRalpha and BCAR3 were expressed by MG63, but estrogen effects in ERbeta-silenced cells were small, so these proteins are either minor regulators in MG63 cells, or act in concert with stimuli in addition to estrogen. We conclude that, in the MG63 cell line, estrogen increases synthesis of matrix proteins via ERbeta, and that, in the absence of additional stimuli, these cells are not major mediators of estrogen effects on osteoclast differentiation. Further, ERalpha is probably much more important in earlier stages of skeletal development, such as growth plate response, than in osteoblasts.

Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells. Publishing Authors By Initials

L Cao,R Bu,JI Oakley,SE Kalla,HC Blair,

For similar peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research abstracts see: peptides: intercellular signaling peptides and proteins: cytokines: transforming growth factor beta research

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Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of cellular biochemistry

VOLUME: 89

Page Numbers: 152-64

Journal Abbreviation: J. Cell. Biochem.

ISSN: 0730-2312

DAY: 1

MONTH: May

YEAR: 2003

Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8205768

Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells. Keywords Mesh Terms:

KEYWORDS: Transforming Growth Factor beta

MESH TERMS: metabolism

Chemical & Substance for Abstract: Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells. Information

Substance Name: Estradiol

Registry Number: 50-28-2

Grant and Affiliation Information for Estrogen receptor-beta modulates synthesis of bone matrix proteins in human osteoblast-like MG63 cells.

AFFILIATION: Department of Pathology, University of Pittsburgh School of Medicine, PA 15243, USA.

Country: United States

AGENCY: United States NIAMS

GRANT: AR47700

ACRONYM: AR

MEDLINETA: J Cell Biochem

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