Ethanolic extracts of four Chinese medicinally used Vitex species were selected and tested for their estrogen-like activities, using an ERalpha-positive MCF-7 cell based proliferation assay (E-screen assay) and cell cycle analysis (flow cytometry). Vitex negundo displayed the highest estrogenic-like activity, and could be useful in hormone replacement therapy (HRT).
Estrogen-like activities in Vitex species from China determined by a cell based proliferation assay. Publishing Authors By Initials