[Establishment of model with inner ear mimetic aging and mtDNA 4834 bp deletion in rats]

[Establishment of model with inner ear mimetic aging and mtDNA 4834 bp deletion in rats] Research Abstract Details 

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[Establishment of model with inner ear mimetic aging and mtDNA 4834 bp deletion in rats] Abstract Text:

Weijia Kong,Yujuan Hu,Qiong Wang,Li Xu,Ying Wang,Yuechen Han,Jun Li,Bo Liu,Wen Kong,

OBJECTIVE: To explore the establishment of the mimetic aging effect in the inner ear of the rat. METHOD: Twenty-four Wistar rats were randomly divided into two groups: group A (D-galactose group, n = 14), which were treated with hypodermic 5% D-galactose (150 mg x kg(-1) x d(-1)) for 8 weeks and then with intraperitoneal saline for 10 days, and group B (control group, n = 10), which were given saline only. Auditory brainstem response(ABR) was used to detect the hearing threshold of rats, and colorimetry was used to analyze the activity of the glutathione peroxidase (GSH-PX). The inner ear tissue was harvested and mitochondrial DNA was amplified to identify the 4 834 bp deletion mutation by nested primer polymerase chain reaction (nested PCR) technique. RESULT: The incidence of mitochondrial DNA 4 834 bp deletion of group A was 100% (28/28), while the deletion of group B was 0. The activity of the GSH-PX in group A (59.07 +/- 8.70 U) was lower than that in group B (142.10 +/- 7.02 U). The difference between group A and group B was significant (t-test, P < 0.01). Variation in ABR threshold of (5.36 +/- 3.08) dB peSPL was observed in group A, and (6.50 +/- 3.37) dB peSPL in group B. The difference in shift of ABR threshold between group A and group B was not significant (P > 0.05). CONCLUSION: The mimetic aging effect in the inner ear of the rat can be induced by D-galactose, and this rat model presents high incidence of mtDNA4834 deletion. But no hearing loss was found in this model.

[Establishment of model with inner ear mimetic aging and mtDNA 4834 bp deletion in rats] Publishing Authors By Initials

W Kong,Y Hu,Q Wang,L Xu,Y Wang,Y Han,J Li,B Liu,W Kong,

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[Establishment of model with inner ear mimetic aging and mtDNA 4834 bp deletion in rats] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Lin chuang er bi yan hou ke za zhi = Journal of cl

VOLUME: 20

Page Numbers: 888-90, 893

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ISSN: 1001-1781

DAY: 15

MONTH: Oct

YEAR: 2006

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LANGUAGE: chi

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AFFILIATION: Department of Otorhinolaryngology, Union Hospital of Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430022, China. wjkong888@yahoo.com

Country: China

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MEDLINETA: Lin Chuang Er Bi Yan Hou Ke Za

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