[Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping]

[Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping] Research Abstract Details 

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[Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping] Abstract Text:

Y Xie,J Hans,G Peng,Y Chen,L Chen,

OBJECTIVE: To establish EBV-immortalized lymphoblast cell lines from Chinese Fanconi anemia (FA) patients. METHODS: The establishment of lymphoblast cell lines was by EBV-immortalization and subtyping of the cell lines by cell fusion, mytomycin C (MMC) inhibition analysis and/or immunoprecipitation and Western blot. RESULT: (1) Three lymphoblast cell lines were established, two of them were sensitive to MMC with a IC (50) < 10 nmol/L. (2) The subtypes of all the three patients belong to FA-A group. CONCLUSION: Complementation analysis was suitable for all FA patients with MMC-sensitive lymphoblastic cell lines, but this method is time consuming. Immunoprecipitation and Western blot are much faster for subtyping but could only be used for FA patients whose related genes had been cloned and specific antibodies been developed.

[Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping] Publishing Authors By Initials

Y Xie,J Hans,G Peng,Y Chen,L Chen,

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[Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zaz

VOLUME: 22

Page Numbers: 173-6

Journal Abbreviation: Zhonghua Xue Ye Xue Za Zhi

ISSN: 0253-2727

DAY: 5

MONTH: Apr

YEAR: 2001

[Establishment of EBV-immortalized lymphoblast cell lines from three Chinese Fanconi anemia patient and their subtyping] Information

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LANGUAGE: chi

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AFFILIATION: Department of Hematology, The First Affiliated Hospital of Hubei Medical University, Wuhan 430060, China.

Country: China

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MEDLINETA: Zhonghua Xue Ye Xue Za Zhi

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