[Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis.]

[Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis.] Research Abstract Details 

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[Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis.] Abstract Text:

Jie Yu,Jing Ye,Fang-ting Zhang,Hui-juan Wan,Mei-jun Yin,Jia-zhi Fang,Yao-ting Gui,Zhi-ming Cai,

OBJECTIVE: To establish an in vitro model for the development of mouse spermatogenic cells into sperm by using the immunodefective mouse as the incubator. METHODS: Tissue grafting was performed using testis from 1-2 days old Kun-ming mice as donor tissue and immunodefective mice as recipients; the expression of TESK1 mRNA in grafts was determined by RT-PCR and the spermatogenesis further observed with histological analysis of grafts. RESULTS: Molecular biological and histological analyses showed that grafts post-grafting not only expressed TESK1 mRNA as in normal mouse testis, but also exhibited similarities in the structure of seminiferous tubules and component of spermatogenic cells, including sperms. CONCLUSION: Spermatogenic cells heterotopically grafted in vitro could continuously grow and complete spermatogenesis and finally develop into sperm.

[Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis.] Publishing Authors By Initials

J Yu,J Ye,FT Zhang,HJ Wan,MJ Yin,JZ Fang,YT Gui,ZM Cai,

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[Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis.] Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Beijing da xue xue bao. Yi xue ban = Journal of Pe

VOLUME: 36

Page Numbers: 591-4

Journal Abbreviation: Beijing Da Xue Xue Bao

ISSN: 1671-167X

DAY: 17

MONTH: Dec

YEAR: 2004

[Establishment of animal model for in vitro spermatogenesis by heterotopic grafting of neonatal mouse testis.] Information

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LANGUAGE: chi

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AFFILIATION: Laboratory of Male Reproduction, Center of Reproductive Medicine, Peking University Shenzhen Hospital, Shenzhen 518036, China. yujie007@hotmail.com

Country: China

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MEDLINETA: Beijing Da Xue Xue Bao

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