[Establishment of a sandwich ELISA for detecting serum sTRAIL and its application]

[Establishment of a sandwich ELISA for detecting serum sTRAIL and its application] Research Abstract Details 

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[Establishment of a sandwich ELISA for detecting serum sTRAIL and its application] Abstract Text:

Ying-Na Li,Yi Li,Xue-Song Liu,Yong Zhu,Xiao-Guang Xu,Bo-Quan Jin,

AIM: To establish quantitative ELISA for detecting souble TRAIL and to evaluate its clinical application. METHODS: The mAb FMU1.1 against TRAIL was used as coating antibody, and the rabbit polyclonal antibody to TRAIL as a sandwich antibody. The serum sTRAIL levels of nine patients with hemorrhagic fever with renal syndrome and forty psoriasis patients were measured by this method. RESUITS: Sandwich ELISA was established by combination of mAb and pAb. The sensitivity of the ELISA was 0.08 microg/L. The increased levels of serum sTRAIL were found in three cases out of nine HFRS patients and eight cases out of forty psoriasis patients. CONCLUSION: A sandwich ELISA is developed, which provide an useful tool judging the patients condition, curable effect and prognosis of some related diseases.

[Establishment of a sandwich ELISA for detecting serum sTRAIL and its application] Publishing Authors By Initials

YN Li,Y Li,XS Liu,Y Zhu,XG Xu,BQ Jin,

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[Establishment of a sandwich ELISA for detecting serum sTRAIL and its application] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese jour

VOLUME: 19

Page Numbers: 190-1

Journal Abbreviation: Xi Bao Yu Fen Zi Mian Yi Xue Z

ISSN: 1007-8738

DAY: 20

MONTH: Mar

YEAR: 2003

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LANGUAGE: chi

NlmUniqueID: 101139110

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AFFILIATION: Department of Immunology, Fourth Military Medical University, Xi'an 710032, China.

Country: China

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MEDLINETA: Xi Bao Yu Fen Zi Mian Yi Xue Z

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