Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice.

Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. Research Abstract Details 

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Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. Abstract Text:

Nobuhiro Kamiya,Akitoshi Jikko,Koji Kimata,Caroline Damsky,Katsuji Shimizu,Hideto Watanabe,

We established a clonal chondrocytic cell line N1511 derived from rib cartilage of a p53-null mouse. N1511 cells proliferated in polygonal shape and elicited differentiation at confluence when treated with combination of bone morphogenetic protein (BMP) 2 and insulin or parathyroid hormone (PTH) and dexamethasone. BMP-2/insulin-treated cells became refractile without forming cartilaginous nodules and reached terminal differentiation, became positive for alizarin red staining, and developed considerable ALP activity. In contrast, PTH/dexamethasone-treated cells formed Alcian blue-positive nodules but remained negative for alizarin red staining and ALP activity. Northern blot analysis revealed that BMP-2/insulin-treated cells sequentially expressed type II, IX, and X collagens, whereas PTH/dexamethasone-treated cells slowly expressed type II collagen and then type IX, and they did not exhibit type X collagen expression. These results show that BMP-2/insulin treatment induces full differentiation toward hypertrophy, whereas treatment with PTH/dexamethasone slows and limits differentiation. Recovery of p53 expression in N1511 cells by transient transfection inhibited cell proliferation, suggesting that cell proliferation could be regulated with p53 in this cell line. These results indicate that N1511 is the only cell line with known genetic mutation, which undergoes multiple steps of chondrocyte differentiation toward hypertrophy, and because proliferation could be regulated by expression of p53, N1511 could be an excellent model for studies of chondrogenesis, the function of p53, and genetic engineering of cartilage tissue.

Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. Publishing Authors By Initials

N Kamiya,A Jikko,K Kimata,C Damsky,K Shimizu,H Watanabe,

For similar proteins: dna-binding proteins: tumor suppressor protein p53 research abstracts see: proteins: dna-binding proteins: tumor suppressor protein p53 research

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Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of bone and mineral research : the officia

VOLUME: 17

Page Numbers: 1832-42

Journal Abbreviation: J. Bone Miner. Res.

ISSN: 0884-0431

DAY: 19

MONTH: Oct

YEAR: 2002

Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8610640

Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. Keywords Mesh Terms:

KEYWORDS: Tumor Suppressor Protein p53

MESH TERMS: physiology

Chemical & Substance for Abstract: Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Establishment of a novel chondrocytic cell line N1511 derived from p53-null mice.

AFFILIATION: Institute for Molecular Science of Medicine, Aichi Medical University, Nagakute, Japan.

Country: United States

AGENCY: United States NIDCR

GRANT: P50 DE10306

ACRONYM: DE

MEDLINETA: J Bone Miner Res

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