Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage.

Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage. Abstract Text:

OBJECTIVE: In this study the gastric mucosa of transgenic mice expressing the simian virus 40 large T antigen gene in the parietal cell lineage is used to establish and characterize a new epithelial progenitor cell line. In these mice, proliferation and amplification of preparietal cells preclude their maturation into acid-secreting parietal cells leading to achlorohydria, hyperplasia, dysplasia and eventually gastric adenocarcinoma. MATERIALS AND METHODS: Enzymatically dispersed gastric epithelial cells were cultured, cloned and screened using immunohistochemical methods, for expression of a variety of biomarkers of differentiated pit, parietal, enteroendocrine and neck/zymogenic cells. RESULTS: A biomarker-deficient cell line whose ultrastructural features resembled those of mouse gastric epithelial progenitor cells was established. Treatment with either hydrocortisone or oestrogen significantly enhanced proliferation of these cells, whereas retinoic acid inhibited their growth. No change in differentiation was detected with any of these treatments; however, when these cells were injected subcutaneously into nude mice, they proliferated to form tumours and undergo partial differentiation towards parietal cell lineage. CONCLUSION: This mouse gastric epithelial progenitor cell line could be useful as an in vitro model to study growth properties, proliferation and differentiation of a subpopulation of gastric epithelial progenitor cells and also to study gastric carcinogenesis.

Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cell proliferation

VOLUME: 41

Page Numbers: 310-20

Journal Abbreviation: Cell Prolif.

ISSN: 1365-2184

DAY: 13

MONTH: Apr

YEAR: 2008

Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9105195

Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage.

AFFILIATION: Department of Anatomy, Faculty of Medicine, Kuwait University, Safat, Kuwait.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Cell Prolif

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage Related Publications

• A study of deaf-mutism in Lebanon.
• Acoustic trauma in unprotected airport workers.
• Adaptive image coding with perceptual distortion control.
• Bridging the quasi-static and the physical optics approximations: an elliptic disk case.
• Characterization of breast cancer progression in the rat.
• Consecutive treatments and the "random walk".
• Duloxetine and suicide attempts: a possible relation.
• Duration of type I diabetes affects glucagon and glucose responses to insulin-induced hypoglycemia.
• Electromagnetic wave interactions with dielectric particles. I. Integral equation reformation.
• Establishment of a gastric epithelial progenitor cell line from a transgenic mouse expressing the simian virus 40 large T antigen gene in the parietal cell lineage.
• Facial nerve decompression and grafting. A report of 8 cases.
• Health systems performance assessment.
• Image Registration based on Neural Network and Fourier Transform.
• Issues in psychiatric research and diagnosis.
• Living the mission in a business model of health care. Sisters of Charity Health System uses 'balanced scorecard' to measure operations.
• Locally adaptive perceptual image coding.
• Morphological text extraction from images.
• OTOSCLERSIOS IN LEBANON; A CLINICAL ANALYSIS.
• Prevention of infertility.
• Pseudosarcoma associated with squamous-cell carcinoma of the larynx (report of a case with review of literature).
• Puerperal uterine inversion.
• Ten years experience with laparoscopic adjustable gastric banding.
• What are the actual indications of electroconvulsive therapy?
• [Cognitive therapy]
• [IS THERE A TREATMENT OF CHOICE FOR JUVENILE COXA VARA OR SLIDING EPIPHYSIS OF THE FEMUR.]
• [Infantile anorexia nervosa]
• [Living donor transplantation. Surgical complications]
• [Preservation of roots by the direct method]
• [Selective salpingography and transcervical tubal recanalization: technique, indications, results. Apropos of 53 proceedings]
• [The drama of delinquent youth throughout the world and in Labanon.]