Establishment of a cisplatin-resistant KB cell line and its characterization.

Establishment of a cisplatin-resistant KB cell line and its characterization. Research Abstract Details 

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Establishment of a cisplatin-resistant KB cell line and its characterization. Abstract Text:

Y Hori,K Kawamoto,N Yamazaki,H Kumazawa,T Yamashita,T Kumazawa,

A KB cell line resistant to cisplatin (KB-rc cell) was successfully established by exposing KB cells to a gradually increasing dose of cisplatin in vitro. The concentration of cisplatin required for 50% inhibition of KB-rc cell proliferation was 1.0 micrograms/ml, while that of KB cells was 0.5 micrograms/ml. Studies of KB-rc cell kinetics after treatment with cisplatin, using flow cytometry, showed that the G2M phase block was inhibited on day 3, and that G0G1 phase cells started to increase on day 5. Therefore, cisplatin-resistance is related to inhibition of the G2M phase block. The decrease of KB-rc cell viability with cisplatin was accelerated by the addition of a Ca antagonist (verapamil) and S phase cells increased on day 3. Verapamil may therefore be useful for enhancement of the effect generated by cisplatin on cisplatin-resistant tumor cells.

Establishment of a cisplatin-resistant KB cell line and its characterization. Publishing Authors By Initials

Y Hori,K Kawamoto,N Yamazaki,H Kumazawa,T Yamashita,T Kumazawa,

For similar organic chemicals: amines: ethylamines: phenethylamines: verapamil research abstracts see: organic chemicals: amines: ethylamines: phenethylamines: verapamil research

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Establishment of a cisplatin-resistant KB cell line and its characterization. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta oto-laryngologica. Supplementum

VOLUME: 500

Page Numbers: 142-8

Journal Abbreviation:

ISSN: 0365-5237

DAY: 13

MONTH: 02

YEAR: 1993

Establishment of a cisplatin-resistant KB cell line and its characterization. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370355

Establishment of a cisplatin-resistant KB cell line and its characterization. Keywords Mesh Terms:

KEYWORDS: Verapamil

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Establishment of a cisplatin-resistant KB cell line and its characterization. Information

Substance Name: Verapamil

Registry Number: 52-53-9

Grant and Affiliation Information for Establishment of a cisplatin-resistant KB cell line and its characterization.

AFFILIATION: Department of Otolaryngology, Kansai Medical University, Osaka, Japan.

Country: SWEDEN

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MEDLINETA: Acta Otolaryngol Suppl

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