Establishment and primary application of a mouse model with hepatitis B virus replication.

Establishment and primary application of a mouse model with hepatitis B virus replication. Research Abstract Details 

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Establishment and primary application of a mouse model with hepatitis B virus replication. Abstract Text:

Feng-Jun Liu,Li Liu,Fang He,Su Wang,Tao-You Zhou,Cong Liu,Lin-Yu Deng,Hong Tang,

AIM: To establish a rapid and convenient animal model with hepatitis B virus (HBV) replication. METHODS: A naked DNA solution of HBV-replication-competent plasmid was transferred to BALB/C mice via the tail vein, using a hydrodynamic in vivo transfection procedure. After injection, these mice were sacrificed on d 1, 3, 4, 5, 7 and 10. HBV DNA replication intermediates in the liver were analyzed by Southern blot hybridization. The expression of hepatitis B core antigen (HBcAg) and hepatitis B surface antigen (HBsAg) in the liver was checked by immunohistochemistry. Serum HBsAg and hepatitis B e antigen (HBeAg) was detected by enzyme-linked immunosorbent assay (ELISA). Inhibition of HBV replication was compared in HBV replication model mice treated intraperitoneally with polyinosinic-polytidylin acid (polyIC) or phosphate-buffered saline (PBS). RESULTS: After hydrodynamic in vivo transfection, HBV DNA replication intermediates in the mouse liver were detectable on d 1 and abundant on d 3 and 4, the levels were slightly decreased and remained relatively stable between d 5 and 7, and were almost undetectable on d 10. The expression patterns of HBcAg and HBsAg were similar to that of HBV replication intermediate DNA, except that they reached a peak on d 1 after injection. No obvious differences in HBV DNA replication intermediates were observed in the left, right and middle lobes of the liver. After treatment with polyIC, the level of HBV intermediate DNA in the liver was lower than that in the control mice injected with PBS. CONCLUSION: A rapid and convenient mouse model with a high level of HBV replication was developed and used to investigate the inhibitory effect of polyIC on HBV replication, which provides a useful tool for future functional studies of the HBV genome.

Establishment and primary application of a mouse model with hepatitis B virus replication. Publishing Authors By Initials

FJ Liu,L Liu,F He,S Wang,TY Zhou,C Liu,LY Deng,H Tang,

For similar biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research abstracts see: biological phenomena, cell phenomena, and immunity: biological phenomena: microbiologic phenomena: viral physiology: virus replication research

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Establishment and primary application of a mouse model with hepatitis B virus replication. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: World journal of gastroenterology : WJG

VOLUME: 13

Page Numbers: 5324-30

Journal Abbreviation: World J. Gastroenterol.

ISSN: 1007-9327

DAY: 28

MONTH: Oct

YEAR: 2007

Establishment and primary application of a mouse model with hepatitis B virus replication. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100883448

Establishment and primary application of a mouse model with hepatitis B virus replication. Keywords Mesh Terms:

KEYWORDS: Virus Replication

MESH TERMS: physiology

Chemical & Substance for Abstract: Establishment and primary application of a mouse model with hepatitis B virus replication. Information

Substance Name: Poly I-C

Registry Number: 24939-03-5

Grant and Affiliation Information for Establishment and primary application of a mouse model with hepatitis B virus replication.

AFFILIATION: Center of Infectious Diseases, Division of Molecular Biology of infectious Diseases, National Key Laboratory of Biotherapy (Sichuan University), West China Hospital of Sichuan University, Chengdu 610041, Sichuan Province, China.

Country: China

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MEDLINETA: World J Gastroenterol

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