Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.

Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Abstract Text:

Y Tabuchi,S Ohta,Y Arai,M Kawahara,K Ishibashi,N Sugiyama,T Horiuchi,M Furusawa,M Obinata,H Fuse,N Takeguchi,S Asano,

We produced an immortalized colonic epithelial cell line, MCE301, using fetal mice transgenic for the temperature-sensitive simian virus 40 large T-antigen gene. MCE301 cells showed epithelial-like morphology and maintained tight connections with neighboring cells. The cells grew at a permissive temperature (33 degrees C), but the growth of the cells was significantly prevented at the nonpermissive temperature (39 degrees C). The cells expressed large T-antigen at 33 degrees C but not at 39 degrees C. MCE301 cells were not transformed, as judged by the absence of anchorage-independent growth in soft agar gel and lack of tumor formation in nude mice. Electron microscopic studies showed that the cells formed microvilli-like structures on the cell surface and junctional complexes such as tight junctions and desmosomes between the cells. The cells expressed cytosketal (acidic cytokeratins and actin), basement membrane (laminin and collagen type IV) and junctional complex proteins (ZO-1 and desmoplakin I + II), as judged by specific antibodies. Fetal bovine serum, epidermal growth factor, insulin-like growth factor and insulin significantly increased the cell growth at 33 degrees C. Moreover, MCE301 cells expressed colonic mucin Muc2 mRNA as demonstrated by reverse transcriptase-polymerase chain reaction, indicating that the cells originate from mucus-secreting cells. Alkaline phosphatase, a brush border-associated enzyme, was detected in the cells. Sodium butyrate (2 mM), an inducer of cellular differentiation, markedly elevated alkaline phosphatase activity. Thus, the present mouse colonic epithelial cell line MCE301 possessing these unique characteristics should provide a useful in vitro model of colonic epithelium.

Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Publishing Authors By Initials

Y Tabuchi,S Ohta,Y Arai,M Kawahara,K Ishibashi,N Sugiyama,T Horiuchi,M Furusawa,M Obinata,H Fuse,N Takeguchi,S Asano,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

PUBMED ID PMID:

MEDLINE DATE:

Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Cell structure and function

VOLUME: 25

Page Numbers: 297-307

Journal Abbreviation: Cell Struct. Funct.

ISSN: 0386-7196

DAY: 30

MONTH: Oct

YEAR: 2000

Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7608465

Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: genetics

Chemical & Substance for Abstract: Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Information

Substance Name: Alkaline Phosphatase

Registry Number: EC 3.1.3.1

Grant and Affiliation Information for Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.

AFFILIATION: Molecular Genetics Research Center, Toyama Medical and Pharmaceutical University, Toyama City, Japan. ytabu@ms.toyama-mpu.ac.jp

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Cell Struct Funct

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene Related Publications

• A novel DNA binding protein that recognizes the methylated c-Myc binding motif.
• Adenosine 3' : 5'-monophosphate binding activity and guanosine 3' : 5'-monophosphate binding activity during adrenocorticotropic hormone stimulation in rat adrenal gland.
• Age dependency of mitochondrial DNA decrease differs in different tissues of rat.
• Amino sugar metabolism in the silkworm, Bombyx mori.
• Analysis of human IgA nephropathy-like disease in galactosyltransferase KO mice.
• Ascorbate synthesizing system in rat liver microsomes. I. Gulonolactone-reducible pigment as a prosthetic group of gulonolactone oxidase.
• Carbohydrate content of fibroin and sericin of the silkworm, Bombyx mori.
• Carboxy-terminal modulator protein induces Akt phosphorylation and activation, thereby enhancing antiapoptotic, glycogen synthetic, and glucose uptake pathways.
• Diastereomer-specific effects of double-stranded peptides conjugated with -L-Tyr-L-Phe- or -L-Tyr-D-Phe- residues on tyrosine phosphorylation and inhibition of src(ts)NRK, A431, MCF-7, and DU145 cell growth.
• Effects of antibiotics on adaptive formation of mitochondria in yeast.
• Enhancement of stress-induced synthesis of stress proteins by mastoparan in C6 rat glioma cells.
• Establishment and characterization of a colonic epithelial cell line MCE301 from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene.
• Eukaryotic Initiation Factor (eIF) 1 Carries Two Distinct eIF5-binding Faces Important for Multifactor Assembly and AUG Selection.
• G protein gamma subunits coimmunoprecipitated with antibodies against alpha subunits: identification of major isoforms in cultured cells by silver stain and immunoblotting with conventional transfer procedure.
• Hyponatremic seizure associated with acute respiratory infection.
• Immunohistochemical expression of T, Tn and sialyl-Tn antigens and clinical outcome in human breast carcinoma.
• Immunolocalization of farnesoid X receptor (FXR) in mouse tissues using tissue microarray.
• Impact of ABO incompatibility on engraftment and transfusion requirement after unrelated cord blood transplantation: a single institute experience in Japan.
• Involvement of Gq/11 in both integrin signal-dependent and -independent pathways regulating endothelin-induced neural progenitor proliferation.
• Localization and characterization of protein-protein interaction sites.
• Regulation of gut-derived resistin-like molecule beta expression by nutrients.
• Rheological studies on calcium-sensitive gelation of actin filaments by actinogelin.
• Shielding design calculation for SPring-8 beamlines using STAC8.
• Stable expression of gastric proton pump activity at the cell surface.
• Structural requirements for hemolytic activity of F-glycoprotein of HVJ (Sendai virus) studied by proteolytic dissection.
• Temporally distinctive changes of alternative splicing patterns during myogenic differentiation of C2C12 cells.
• Transrectal high-intensity focused ultrasound for treatment for patients with biochemical failure after radical prostatectomy.
• Unusual variant of persistent primitive hypoglossal artery.
• Yeast phenotypic assays on translational control.
• differential effect of Listeria monocytogenes infection on cytokine production and cytotoxicity of CD8 T cells.