Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins.

Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins. Research Abstract Details 

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Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins. Abstract Text:

Reynolds K B Brobey,Lynn Soong,

The recent completion of genome sequencing projects for Leishmania major and near completion for two other species, L. infantum and L. braziliensis, has provided the needed genomic information for investigating the proteomes of Leishmania parasites. However, the design of effective 2-DE-based proteome mapping for complex protozoan parasites like Leishmania has proven to be severely compromised due to extensive overcrowding of spots especially in the acidic regions, coupled to a relatively low representation of basic proteins. In the present study, we optimized a liquid-phase IEF in combination with 2-DE for L. amazonensis promastigote as a way of reducing protein complexity and enhancing representation for low-abundance proteins on gels. Of 20 pH-based fractions eluted from Rotofor cells, 5 representative fractions selected from acidic, basic or neutral regions of the proteome and with adequate protein concentration were further analyzed by 2-DE using medium-range IPG strips. On this basis, we were able to generate high-resolution 2-DE maps encompassing both the acidic and basic ends of the proteome with enhanced spot representation.

Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins. Publishing Authors By Initials

RK Brobey,L Soong,

For similar proteins: protozoan proteins research abstracts see: proteins: protozoan proteins research

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Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proteomics

VOLUME: 7

Page Numbers: 116-20

Journal Abbreviation: Proteomics

ISSN: 1615-9853

DAY: 3

MONTH: Jan

YEAR: 2007

Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101092707

Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins. Keywords Mesh Terms:

KEYWORDS: Protozoan Proteins

MESH TERMS: analysis

Chemical & Substance for Abstract: Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins. Information

Substance Name: Protozoan Proteins

Registry Number: 0

Grant and Affiliation Information for Establishing a liquid-phase IEF in combination with 2-DE for the analysis of Leishmania proteins.

AFFILIATION: Department of Microbiology and Immunology, Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, TX 77555-1070, USA.

Country: Germany

AGENCY: United States NIAID

GRANT: AI43003

ACRONYM: AI

MEDLINETA: Proteomics

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