Establishing a genetic system for ecological studies of Streptococcus oligofermentans.

Establishing a genetic system for ecological studies of Streptococcus oligofermentans. Research Abstract Details 

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Establishing a genetic system for ecological studies of Streptococcus oligofermentans. Abstract Text:

Huichun Tong,Baoli Zhu,Wei Chen,Fengxia Qi,Wenyuan Shi,Xiuzhu Dong,

Streptococcus oligofermentans is a newly characterized species belonging to the mitis group of oral streptococci. So far no correlation has been demonstrated between S. oligofermentans and dental caries. Furthermore, a reverse correlation has been observed between the number of S. oligofermentans and the number of Streptococcus mutans, a major cariogenic pathogen, in the oral cavity. These properties suggest that S. oligofermentans may have a potential to be used as a 'probiotics' for caries prevention. In this study, we aim to establish a genetic system in S. oligofermentans to further study the biology of this new species. Using homologous regions of the comCDE locus in other streptococci, the comC gene was isolated and sequenced. A synthetic competence-stimulating peptide (CSP) was synthesized and shown to be able to effectively induce competence in S. oligofermentans. This CSP-induced transformation system in S. oligofermentans was used to construct green fluorescent protein (gfp) and luciferase (luc) reporter systems, both of which are driven by the lactate dehydrogenase (ldh) promoter. These reporter systems were further shown to be highly expressed in planktonic and biofilm cells, suggesting that these reporter systems can be used in future ecological studies of S. oligofermentans.

Establishing a genetic system for ecological studies of Streptococcus oligofermentans. Publishing Authors By Initials

H Tong,B Zhu,W Chen,F Qi,W Shi,X Dong,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection: transformation, bacterial research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection: transformation, bacterial research

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Establishing a genetic system for ecological studies of Streptococcus oligofermentans. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: FEMS microbiology letters

VOLUME: 264

Page Numbers: 213-9

Journal Abbreviation: FEMS Microbiol. Lett.

ISSN: 0378-1097

DAY: 3

MONTH: Nov

YEAR: 2006

Establishing a genetic system for ecological studies of Streptococcus oligofermentans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7705721

Establishing a genetic system for ecological studies of Streptococcus oligofermentans. Keywords Mesh Terms:

KEYWORDS: Transformation, Bacterial

MESH TERMS: physiology

Chemical & Substance for Abstract: Establishing a genetic system for ecological studies of Streptococcus oligofermentans. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Establishing a genetic system for ecological studies of Streptococcus oligofermentans.

AFFILIATION: State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.

Country: Netherlands

AGENCY: United States NIDCR

GRANT: R01-DE014757

ACRONYM: DE

MEDLINETA: FEMS Microbiol Lett

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