EST-derived microsatellites from Actinidia species and their potential for mapping.

EST-derived microsatellites from Actinidia species and their potential for mapping. Research Abstract Details 

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EST-derived microsatellites from Actinidia species and their potential for mapping. Abstract Text:

L G Fraser,C F Harvey,R N Crowhurst,H N De Silva,

To increase the speed and reduce the cost of constructing a genetic map of Actinidia species (kiwifruit), for use in both breeding and functional genomics programmes, we sampled microsatellites from expressed sequence tags (ESTs) to evaluate their frequency of occurrence and level of polymorphism. Perfect dinucleotide repeats were the microsatellites selected, and these were found to be numerous in both the 5' and 3' ends of the genes represented. The microsatellites were of various lengths, the majority being repeats with the pattern (CT)(n)/(GA)(n). One hundred and fifty microsatellites, each with more than 10 dinucleotide repeat units, were chosen as possible markers, and when these were amplified, 93.5% were found to be polymorphic and segregating in a mapping population, with 22.6% amplifying more than one locus. Four marker categories were identified. Fully informative markers made up 27% of the total, 36.2% were female informative, 25.8% were male informative and 10% partly informative. The mapping population was an intraspecific cross in the diploid species Actinidia chinensis, with parents chosen for their diversity in fruit and plant characteristics, and for their geographical separation. Linkage was tested using the software 'Joinmap' and a LOD value of 3. The distribution of the EST-based markers over the linkage groups obtained appeared to be random, taking into consideration the small sample size, that the number of linkage groups (31) exceeded the chromosome number of n=29, and that a number of markers were not assigned to any group. Some microsatellite markers which amplified more than one locus mapped to separate linkage groups. According to our study in A. chinensis, EST-derived microsatellites give large numbers of possible markers very quickly and at reasonable cost. The markers are highly polymorphic, segregate in the mapping population, and increase the value of the genomic map by providing some functional information.

EST-derived microsatellites from Actinidia species and their potential for mapping. Publishing Authors By Initials

LG Fraser,CF Harvey,RN Crowhurst,HN De Silva,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: repetitive sequences, nucleic acid: tandem repeat sequences: microsatellite repeats research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: repetitive sequences, nucleic acid: tandem repeat sequences: microsatellite repeats research

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EST-derived microsatellites from Actinidia species and their potential for mapping. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 108

Page Numbers: 1010-6

Journal Abbreviation:

ISSN: 0040-5752

DAY: 20

MONTH: 11

YEAR: 2003

EST-derived microsatellites from Actinidia species and their potential for mapping. Information

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LANGUAGE: eng

NlmUniqueID: 145600

EST-derived microsatellites from Actinidia species and their potential for mapping. Keywords Mesh Terms:

KEYWORDS: Microsatellite Repeats

MESH TERMS: genetics

Chemical & Substance for Abstract: EST-derived microsatellites from Actinidia species and their potential for mapping. Information

Substance Name: DNA Primers

Registry Number: 0

Grant and Affiliation Information for EST-derived microsatellites from Actinidia species and their potential for mapping.

AFFILIATION: The Horticulture and Food Research Institute of New Zealand, 120 Mt Albert Road, Auckland, New Zealand. lfraser@hortresearch.co.nz

Country: Germany

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MEDLINETA: Theor Appl Genet

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