Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses.

Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses. Abstract Text:

Manchao Zhang,Youping Deng,Ruchi Tandon,Cheng Bai,Heimo Riedel,Manchao Zhang,Youping Deng,Ruchi Tandon,Cheng Bai,Heimo Riedel,

The positive regulatory role of PSM/SH2-B downstream of various mitogenic receptor tyrosine kinases or gene disruption experiments in mice support a role of PSM in the regulation of insulin action. Here, four alternative PSM splice variants and individual functional domains were compared for their role in the regulation of specific metabolic insulin responses. We found that individual PSM variants in 3T3-L1 adipocytes potentiated insulin-mediated glucose and amino acid transport, glycogenesis, lipogenesis, and key components in the metabolic insulin response including p70 S6 kinase, glycogen synthase, glycogen synthase kinase 3 (GSK3), Akt, Cbl, and IRS-1. Highest activity was consistently observed for PSM alpha, followed by beta, delta, and gamma with decreasing activity. In contrast, dominant-negative peptide mimetics of the PSM Pro-rich, pleckstrin homology (PH), or src homology 2 (SH2) domains inhibited any tested insulin response. Potentiation of the insulin response originated at the insulin receptor (IR) kinase level by PSM variant-specific regulation of the K(m) (ATP) whereas the V(max) remained unaffected. IR catalytic activation was inhibited by peptide mimetics of the PSM SH2 or dimerization domain (DD). Either peptide should disrupt the complex of a PSM dimer linked to IR via SH2 domains as proposed for PSM activation of tyrosine kinase JAK2. Either peptide abolished downstream insulin responses indistinguishable from PSM siRNA knockdown. Our results implicate an essential role of the PSM variants in the activation of the IR kinase and the resulting metabolic insulin response. PSM variants act as internal IR ligands that in addition to potentiating the insulin response stimulate IR catalytic activation even in the absence of insulin. J. Cell. Biochem. 103: 162-181, 2008. (c) 2007 Wiley-Liss, Inc.

Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses. Publishing Authors By Initials

M Zhang,Y Deng,R Tandon,C Bai,H Riedel,M Zhang,Y Deng,R Tandon,C Bai,H Riedel,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of cellular biochemistry

VOLUME: 103

Page Numbers: 162-81

Journal Abbreviation: J. Cell. Biochem.

ISSN: 0730-2312

DAY: 1

MONTH: Jan

YEAR: 2008

Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8205768

Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses.

AFFILIATION: Department of Biochemistry, West Virginia University, School of Medicine, Morgantown, West Virginia 26506-9142.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Cell Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses Related Publications

• "I thought there was no hope for me": a behavioral intervention for urban mothers with problem drinking.
• An examination of oil in water ointment bases.
• Clinical evaluation of recombinant human bone morphogenetic protein-2.
• Deletion analysis of protein kinase Calpha reveals a novel regulatory segment.
• Design and start-up of a high rate anaerobic membrane bioreactor for the treatment of a low pH, high strength, dissolved organic waste water.
• Essential role of PSM/SH2-B variants in insulin receptor catalytic activation and the resulting cellular responses.
• Fibrinolytic treatment with ultra-high streptokinase infusion via the dorsalis pedis vein offers no advantage over systemic infusion via the brachial vein in patients with deep vein thrombosis of the leg.
• Ligand regulation of bovine protein kinase C alpha response via either cysteine-rich repeat of conserved region C1.
• Milk as a source of some protection against the acquisition of Trichinella spiralis in mice.
• Ossifying fibromyxoid tumor of the nasal septum.
• PSM/SH2-B distributes selected mitogenic receptor signals to distinct components in the PI3-kinase and MAP kinase signaling pathways.
• Plastic films in medicine.
• Tissue engineering in head and neck reconstructive surgery: what type of tissue do we need?
• Universal oil-in-water ointment base.
• Using a health and productivity dashboard: a case example.
• ["Baby cradle coop slots" for abandoned (anonymous) babies: legal problems]
• [CYSTS AND TUMORS OF THE EXTERNAL GENITALIA AND THE VAGINA.]
• [Chronic recurrent abdominal pain--significance and success of laparoscopic/pelviscopic adhesiolysis]
• [Clinical and endocrinologic studies of the treatment of ovarian insufficiency manifestations following hysterectomy with intact adnexa]
• [Health inspector & veterinary public health in Brazil.]
• [In-vitro rates of fertilization and culture to blastocyst stage of mouse oocytes following co-incubation of murine spermatozoa with Escherichia coli and Ureaplasma urealyticum]
• [Incidence distribution of postoperative adhesions after using different coagulation techniques. An animal experiment study]
• [Multiple phlebothrombosis following cesarean section due to heterozygous factor V Leiden mutation detected post partum]
• [Not Available.]
• [Pelviscopic treatment of tubal pregnancy]
• [Possibilities and limits of mammography with special reference to breast carcinoma--a comparison of clinical, mammography and histologic diagnoses]
• [Possibilities and significance of using lasers in gynecology]
• [Retrospective study of pelviscopic adhesiolysis for treatment of chronic lower abdominal pain (January 1996-December 1997)]
• [Symptoms of ovarian failure after hysterectomy in premenopausal women. A retrospective study based on postoperative perception of 245 women]
• [Tubal sterilization in the new unified Germany--results of a survey]