Essential Role of Monocytes in the In Vitro Production of IL-4 and Nonspecific IgE Antibody by Peripheral Blood Lymphocytes from Mice Sensitized s.c. Once with Cedar Pollen.

Essential Role of Monocytes in the In Vitro Production of IL-4 and Nonspecific IgE Antibody by Peripheral Blood Lymphocytes from Mice Sensitized s.c. Once with Cedar Pollen. Research Abstract Details 

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Essential Role of Monocytes in the In Vitro Production of IL-4 and Nonspecific IgE Antibody by Peripheral Blood Lymphocytes from Mice Sensitized s.c. Once with Cedar Pollen. Abstract Text:

Yumiko Yamamoto,Junko Tashiro-Yamaji,Kanji Sakurai,Masako Miyoshi-Higashino,Hayahito Nomi,Sayako Miura-Takeda,Masashi Okada,Satoko Yamaguchi,Hiroshi Takenaka,Takahiro Kubota,Ryotaro Yoshida,Yumiko Yamamoto,Junko Tashiro-Yamaji,Kanji Sakurai,Masako Miyoshi-Higashino,Hayahito Nomi,Sayako Miura-Takeda,Masashi Okada,Satoko Yamaguchi,Hiroshi Takenaka,Takahiro Kubota,Ryotaro Yoshida,Yumiko Yamamoto,Junko Tashiro-Yamaji,Kanji Sakurai,Masako Miyoshi-Higashino,Hayahito Nomi,Sayako Miura-Takeda,Masashi Okada,Satoko Yamaguchi,Hiroshi Takenaka,Takahiro Kubota,Ryotaro Yoshida,

To explore which cytokine or cell is essential for the production of antibodies (Abs) of the IgE class in allergic diseases, we injected cedar pollen into wild-type, interferon-gamma(/) (IFN-gamma(/)), or interleukin-4(/) (IL-4(/)) BALB/c mice through four (i.n., i.p., s.c., and i.v.) different routes without adjuvant. Wild-type or IFN-gamma(/), but not IL-4(/), mice sensitized once or twice showed a significant increase in total IgE Ab in their serum, revealing the essential role of IL-4 in the production of total IgE Ab. We separated peripheral blood mononuclear cells (PBMCs) from untreated or sensitized mice into monocyte-rich, lymphocyte-rich, and granulocyterich populations by Percoll density-gradient centrifugation or into specific antigen cells by flow cytometry, cultured the cells in various combinations, and examined the levels of cytokines and IgE Ab released into the medium. The PBMCs from mice sensitized s.c. once, but not those from untreated animals, produced significant amounts of IL-4 and total IgE Ab, whereas the lymphocyte-rich population alone did not. Unexpectedly, IL-4 and IgE Ab production was restored by the addition of Mac-1(+) cells in the monocyte-rich fraction to the lymphocyte-rich fraction. These results indicate the essential role of monocytes in the production of IL-4 and total IgE Ab by lymphocytes during the initial stage of sensitization.

Essential Role of Monocytes in the In Vitro Production of IL-4 and Nonspecific IgE Antibody by Peripheral Blood Lymphocytes from Mice Sensitized s.c. Once with Cedar Pollen. Publishing Authors By Initials

Y Yamamoto,J Tashiro-Yamaji,K Sakurai,M Miyoshi-Higashino,H Nomi,S Miura-Takeda,M Okada,S Yamaguchi,H Takenaka,T Kubota,R Yoshida,Y Yamamoto,J Tashiro-Yamaji,K Sakurai,M Miyoshi-Higashino,H Nomi,S Miura-Takeda,M Okada,S Yamaguchi,H Takenaka,T Kubota,R Yoshida,Y Yamamoto,J Tashiro-Yamaji,K Sakurai,M Miyoshi-Higashino,H Nomi,S Miura-Takeda,M Okada,S Yamaguchi,H Takenaka,T Kubota,R Yoshida,

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Essential Role of Monocytes in the In Vitro Production of IL-4 and Nonspecific IgE Antibody by Peripheral Blood Lymphocytes from Mice Sensitized s.c. Once with Cedar Pollen. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of interferon & cytokine research : the of

VOLUME: 27

Page Numbers: 1019-30

Journal Abbreviation: J. Interferon Cytokine Res.

ISSN: 1079-9907

DAY: 10

MONTH: Dec

YEAR: 2007

Essential Role of Monocytes in the In Vitro Production of IL-4 and Nonspecific IgE Antibody by Peripheral Blood Lymphocytes from Mice Sensitized s.c. Once with Cedar Pollen. Information

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LANGUAGE: eng

NlmUniqueID: 9507088

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Grant and Affiliation Information for Essential Role of Monocytes in the In Vitro Production of IL-4 and Nonspecific IgE Antibody by Peripheral Blood Lymphocytes from Mice Sensitized s.c. Once with Cedar Pollen.

AFFILIATION: Department of Physiology, Osaka Medical College, Takatsuki 569-8686, Japan., Department of Otorhinolaryngology, Osaka Medical College, Takatsuki 569-8686, Japan.

Country: United States

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MEDLINETA: J Interferon Cytokine Res

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