Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide.

Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Research Abstract Details 

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Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Abstract Text:

Wen Yi,Jun Shao,Lizhi Zhu,Mei Li,Mamata Singh,Yuquan Lu,Steven Lin,Hanfen Li,Kang Ryu,Jie Shen,Hongjie Guo,Qingjia Yao,C Allen Bush,Peng G Wang,

Previous study showed that some Gram-negative bacteria possess human blood group activity. Among them, Escherichia coli O86 has high blood group B activity and weak blood group A activity. This is due to the cell surface O-antigen structure, which resembles that of human blood group B antigen. In this study, we sequenced the entire E. coli O86 antigen gene cluster and identified all the genes responsible for O-antigen biosynthesis by sequence comparative analysis. The blood group B-like antigen in E. coli O86 O-polysaccharide was synthesized by sequentially employing three glycosyltransferases identified in the gene cluster. More importantly, we identified a new bacterial glycosyltransferase (WbnI) equivalent to human blood group transferase B (GTB). The enzyme substrate specificity and stepwise enzymatic synthesis of blood group B-like antigen revealed that the biosynthetic pathway of B antigen is essentially the same in E. coli O86 as in humans. This new finding provides a model to study the specificity and structure relationship of blood group transferases and supports the hypothesis of anti-blood group antibody production by bacterial stimulation.

Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Publishing Authors By Initials

W Yi,J Shao,L Zhu,M Li,M Singh,Y Lu,S Lin,H Li,K Ryu,J Shen,H Guo,Q Yao,CA Bush,PG Wang,

For similar carbohydrates: polysaccharides: oligosaccharides research abstracts see: carbohydrates: polysaccharides: oligosaccharides research

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Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 127

Page Numbers: 2040-1

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 23

MONTH: Feb

YEAR: 2005

Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Keywords Mesh Terms:

KEYWORDS: Oligosaccharides

MESH TERMS: genetics

Chemical & Substance for Abstract: Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide. Information

Substance Name: Glycosyltransferases

Registry Number: EC 2.4.-

Grant and Affiliation Information for Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide.

AFFILIATION: Department of Biochemistry, The Ohio State University, Columbus, Ohio 43210, USA.

Country: United States

AGENCY: United States NIAID

GRANT: R01 AI44040

ACRONYM: AI

MEDLINETA: J Am Chem Soc

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