Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III.

Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Research Abstract Details 

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Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Abstract Text:

Feng Li,Qin Liu,Yuan-Yuan Chen,Zi-Niu Yu,Zhi-Ping Zhang,Ya-Feng Zhou,Jiao-Yu Deng,Li-Jun Bi,Xian-En Zhang,

It has been hypothesized that DNA mismatch repair (MMR) is coupled with DNA replication; however, the involvement of DNA polymerase III subunits in bacterial DNA MMR has not been clearly elucidated. In an effort to better understand the relationship between these 2 systems, the potential interactions between the Escherichia coli MMR protein and the clamp loader subunits of E. coli DNA polymerase III were analyzed by far western blotting and then confirmed and characterized by surface plasmon resonance (SPR) imaging. The results showed that the MMR key protein MutL could directly interact with both the individual subunits delta, delta', and gamma and the complex of these subunits (clamp loader). Kinetic parameters revealed that the interactions are strong and stable, suggesting that MutL might be involved in the recruitment of the clamp loader during the resynthesis step in MMR. The interactions between MutL, the delta and gamma subunits, and the clamp loader were observed to be modulated by ATP. Deletion analysis demonstrated that both the N-terminal residues (1-293) and C-terminal residues (556-613) of MutL are required for interacting with the subunits delta and delta'. Based on these findings and the available information, the network of interactions between the MMR components and the DNA polymerase III subunits was established; this network provides strong evidence to support the notion that DNA replication and MMR are highly associated with each other.

Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Publishing Authors By Initials

F Li,Q Liu,YY Chen,ZN Yu,ZP Zhang,YF Zhou,JY Deng,LJ Bi,XE Zhang,

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Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Mutation research

VOLUME: 637

Page Numbers: 101-10

Journal Abbreviation: Mutat. Res.

ISSN: 0027-5107

DAY: 25

MONTH: 07

YEAR: 2007

Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Escherichia coli mismatch repair protein MutL interacts with the clamp loader subunits of DNA polymerase III.

AFFILIATION: State Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan 430070, China(1).

Country: Netherlands

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MEDLINETA: Mutat Res

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