Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus.

Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus. Abstract Text:

AIM: Infants born prematurely lack a normal response to anemia and fail to increase erythropoietin (Epo) production despite an apparent need for improved tissue oxygenation. This anemia may involve a deficiency in the fetal and premature kidney to produce Epo. To evaluate fetal Epo production, Epo and hypoxia inducible factor-1 (HIF) mRNA expression was measured in the mid-trimester human fetus. METHODS: Fetal liver and kidney samples were obtained at 11-22 wk of gestation. RNA was isolated and reverse transcribed from snap-frozen specimens. Epo and HIF cDNA concentrations were determined using real-time polymerase chain reaction (PRISM). Epo cDNA concentrations were standardized to HIF concentrations present in each sample. RESULTS: HIF concentrations remained constant during gestation in kidney and liver samples. Epo cDNA concentrations in kidney did not change from 12 to 22 wk (8.4 +/- 3.4 fg Epo pg(-1) HIF cDNA, 4.8 +/- 1.4, 2.6 +/- 0.4, and 4.2 +/- 1.8 at 11-14, 15-16, 17-19, and 20-22 wk of gestation, respectively), while Epo cDNA concentrations in liver increased with gestation (74.5 +/- 31.9 fg pg(-1) HIF, 23.8 +/- 6.5, 96.4 +/- 19.2 and 276.1 +/- 28.5 at 12-14, 15-16, 17-19 and 20-22 wk of gestation, respectively, p < 0.05, 20-22 wk of gestation liver samples vs all other gestations). Concentrations were 5-20-fold higher in liver than in kidney in each gestational group (p < 0.01, liver vs kidney). CONCLUSION: HIF concentrations did not change with gestation in liver or kidney. The human fetal kidney produced approximately 5% of the total Epo mRNA measured during the second trimester. It remains to be determined how Epo production by these tissues is affected by premature birth.

Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus. Publishing Authors By Initials

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

PUBMED ID PMID:

MEDLINE DATE:

Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta paediatrica (Oslo, Norway : 1992). Supplement

VOLUME: 91

Page Numbers: 27-30

Journal Abbreviation: Acta Paediatr Suppl

ISSN: 0803-5326

DAY: 12

MONTH: 05

YEAR: 2002

Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9315043

Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: analysis

Chemical & Substance for Abstract: Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus. Information

Substance Name: Erythropoietin

Registry Number: 11096-26-7

Grant and Affiliation Information for Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus.

AFFILIATION: Department of Pediatrics, Division of Neonatology, University of New Mexico School of Medicine, Albuquerque, New Mexico 87131-5311, USA. rohls@unm.edu

Country: Norway

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Acta Paediatr Suppl

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus Related Publications

• Elevated erythropoietin mRNA and protein concentrations in the developing human eye.
• Erythropoietin and hypoxia inducible factor-1 expression in the mid-trimester human fetus.