ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans.

ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans. Abstract Text:

Yohei Sasagawa,Kunitoshi Yamanaka,Teru Ogura,

p97 (also called VCP or Cdc48p) and E3 ubiquitin ligases are the key players in retrotranslocation and ubiquitination of substrates in the endoplasmic reticulum-associated degradation (ERAD) pathways. Although their biochemical properties have been well studied, their cellular functions in development have not been revealed. Here, we investigate cellular functions of p97 and E3 ubiquitin ligases in Caenorhabditis elegans as a model organism. We found that C. elegans possesses three E3 ubiquitin ligases (named as HRD-1, HRDL-1 and MARC-6) like mammals, and that their simultaneous depletion caused extremely delayed growth. By monitoring the expression of an ER chaperone gene, it was revealed that p97 and HRD-1 play essential roles in unfolded protein response (UPR) and ERAD pathways. We further found that HRD-1 functions in concert with BiP, and that two BiP paralogues are functionally diversified. HRD-1 and BiP(HSP-3) play important roles in the developmental growth and function of intestinal cells, while HRD-1 and BiP(HSP-4) in the gonad formation. We propose that E3 ubiquitin ligases function in concert with a specific partner chaperone.

ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans. Publishing Authors By Initials

Y Sasagawa,K Yamanaka,T Ogura,

For similar enzymes and coenzymes: enzymes: ligases: ubiquitin-protein ligase complexes: ubiquitin-protein ligases research abstracts see: enzymes and coenzymes: enzymes: ligases: ubiquitin-protein ligase complexes: ubiquitin-protein ligases research

PUBMED ID PMID:

MEDLINE DATE:

ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Genes to cells : devoted to molecular & cellular m

VOLUME: 12

Page Numbers: 1063-73

Journal Abbreviation: Genes Cells

ISSN: 1356-9597

DAY: 27

MONTH: Sep

YEAR: 2007

ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9607379

ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans. Keywords Mesh Terms:

KEYWORDS: Ubiquitin-Protein Ligases

MESH TERMS: physiology

Chemical & Substance for Abstract: ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans. Information

Substance Name: Ubiquitin-Protein Ligases

Registry Number: EC 6.3.2.19

Grant and Affiliation Information for ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans.

AFFILIATION: Division of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Genes Cells

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans Related Publications

• Action mechanism of glucose oxidase of Aspergillus niger.
• Action mechanism of tyramine oxidase from Sarcina lutea.
• Basic research for Alzheimer's disease drugs.
• ER E3 ubiquitin ligase HRD-1 and its specific partner chaperone BiP play important roles in ERAD and developmental growth in Caenorhabditis elegans.
• Effect of pH on the kinetic parameters of yeast L(plus)-lactate dehydrogenase (cytochrome b2).
• Effect of pH on the optical absorption spectrum and effective number of the Bohr magneton of horse erythrocyte catalase in the range from 77 degrees K to 267 degrees K.
• Effect of the alterations in the fusion protein of measles virus isolated from brains of patients with subacute sclerosing panencephalitis on syncytium formation.
• Effects of urea on the activity and structure of yeast alcohol dehydrogenase.
• Efficient production of intersubspecific hybrid mice and embryonic stem cells by intracytoplasmic sperm injection.
• Electron paramagnetic resonance study of bovine liver catalase.
• Glycoxidized particles mimic lipofuscin accumulation in aging eyes: a new age-related macular degeneration model in rabbits.
• Hydrolysis of some synthetic peptides catalyzed by Bacillus thermoproteolytics protease.
• Kinetic studies on the action of collagenase.
• Magnetic susceptibility and EPR measurements of catalase and its derivatives. A thermal equilibrium between the high- and low-spin states in the catalase-azide compound.
• Mode of inhibition of glucose oxidase by metal ions.
• Mode of inhibition of the Aspergillus amine oxidase by 8-hydroxyquinoline.
• On the purification and properties of mushroom tyrosinase.
• Oxidation and reduction of copper proteins: note added to the previous report on the state and activity of copper atoms in copper proteins.
• Oxidation-reduction titrations of copper ions in Rhus-laccase. Properties of two types of copper ions in the molecule.
• Purification and properties of ascorbate oxidase from cucumber.
• Radiological and histological features of tumoral calcinosis of the dorsum of the foot.
• Spectrophotometric and electron spin resonance studies of NADH2-cytochrome c reductase complex.
• Steroids as substrates for cholesterol: oxygen oxidoreductase, with special reference to 3beta-hydroxy bile acids.
• Structural transitions of bovine plasma albumin. Location of tyrosyl and tryptophyl residues by solvent perturbation difference spectra.
• Studies on the EPR and light absorption spectra of horse erythrocyte catalase and its derivatives.
• Studies on the respiratory system of Aspergillus oryzae. IV. Low temperature difference spectra of mitochondria from mycelia grown in the presence and absence of chloramphenicol.
• The kinetic behavior of the FMN and protoheme moieties of yeast L(plus)-lactate dehydrogenase (cytochrome b2).
• The nature of the copper in Rhus vernicifera laccase.
• The state of copper in Rhus laccase as compared with those in other copper complexes.
• [Current status and future perspective of new drugs for hematological malignancies in Japan]