Equilibrium unfolding of the poly(glutamic acid)20 helix.

Equilibrium unfolding of the poly(glutamic acid)20 helix. Research Abstract Details 

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Equilibrium unfolding of the poly(glutamic acid)20 helix. Abstract Text:

John M Finke,Patricia A Jennings,Jennifer C Lee, Onuchic,Jay R Winkler,

The equilibrium structural ensemble of a 20-residue polyglutamic acid peptide (E(20)) was studied with FRET, circular dichroism, and molecular dynamics (MD) simulations. A FRET donor, o-aminobenzamide, and acceptor, 3-nitrotyrosine, were introduced at the N- and C-termini, respectively. Circular dichroism, steady state FRET, and time-resolved FRET measurements were employed to characterize the fraction helix and end-to-end distance under different pH conditions: pH 4 (60% alpha-helix), pH 6 (0% alpha-helix), and pH 9 (0% alpha-helix). At pH 4, the end-to-end distance was measured at 24 A and determined to be considerably less than the 31 A predicted for an alpha-helix of the same length. At pH 6 and 9, the end-to-end distance was measured at > 31 and 39 A respectively, both which are determined to be considerably greater than the 27 A predicted for a freely jointed random coil of the same length. To better understand the physical forces underlying the unusual helix-coil transition in this peptide, three theoretical MD models of E(20) were constructed: (1) a pure alpha-helix, (2) an alpha-helix with equivalent attractive intramolecular contacts, and (3) a weak alpha-helix with termini-weighted intramolecular contacts ("sticky ends"). Using MD simulations, the bent helix structure calculated from Model 3 was found to be the closest in agreement with the experimental data.

Equilibrium unfolding of the poly(glutamic acid)20 helix. Publishing Authors By Initials

JM Finke,PA Jennings,JC Lee,JN Onuchic,JR Winkler,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

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Equilibrium unfolding of the poly(glutamic acid)20 helix. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Biopolymers

VOLUME: 86

Page Numbers: 193-211

Journal Abbreviation: Biopolymers

ISSN: 0006-3525

DAY: 15

MONTH: Jun

YEAR: 2007

Equilibrium unfolding of the poly(glutamic acid)20 helix. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 372525

Equilibrium unfolding of the poly(glutamic acid)20 helix. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: chemistry

Chemical & Substance for Abstract: Equilibrium unfolding of the poly(glutamic acid)20 helix. Information

Substance Name: Polyglutamic Acid

Registry Number: 25513-46-6

Grant and Affiliation Information for Equilibrium unfolding of the poly(glutamic acid)20 helix.

AFFILIATION: Department of Chemistry, Oakland University, Rochester, MI 48309-4477, USA. finke@oakland.edu

Country: United States

AGENCY: United States NIGMS

GRANT: GM54038

ACRONYM: GM

MEDLINETA: Biopolymers

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