Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies.

Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies. Abstract Text:

Hepatitis B surface antigen (HBsAg) induces a potent protective antibody response in immunized healthy individuals. The antibody response in humans is largely directed to a restricted conformational immunodominant region of HBsAg, identified as "a" determinant. Our aim was generation and characterization of murine monoclonal antibodies (MAbs) against recombinant HBsAg and their use for epitope mapping of the antigen. Hybridoma cells were established from Balb/c mice immunized with recombinant HBsAg of the "adw" subtype and cloned by limiting dilution. Specificity of MAbs was studied by indirect ELISA and immunoblotting. Topology of the epitopes was analyzed by competitive and inhibition ELISA. Eight hybridoma clones producing MAbs specific for the immunogen were established. Five of the MAbs recognized overlapping conformational epitopes, whereas the remaining three MAbs were found to identify linear epitopes. Cross-inhibition studies suggest recognition of mutually exclusive epitopes by these MAbs. Our data suggest that, similar to the human system, the mouse antibody response is largely directed to restricted conformational overlapping epitopes of HBsAg.

Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies. Publishing Authors By Initials

For similar proteins: recombinant proteins research abstracts see: proteins: recombinant proteins research

PUBMED ID PMID:

MEDLINE DATE:

Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Hybridoma (2005)

VOLUME: 24

Page Numbers: 71-7

Journal Abbreviation: Hybridoma (Larchmt)

ISSN: 1554-0014

DAY: 28

MONTH: Apr

YEAR: 2005

Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101241539

Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies. Keywords Mesh Terms:

KEYWORDS: Recombinant Proteins

MESH TERMS: immunology

Chemical & Substance for Abstract: Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies. Information

Substance Name: Recombinant Proteins

Registry Number: 0

Grant and Affiliation Information for Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies.

AFFILIATION: Department of Immunology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Hybridoma (Larchmt)

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies Related Publications

• Cross-sectional monitoring of Wilms' tumor gene 1 (WT1) expression in Iranian patients with acute lymphoblastic leukemia at diagnosis, relapse and remission.
• Differential expression of rheumatoid factor-associated crossreactive idiotypes in Iranian seropositive and seronegative patients with rheumatoid arthritis.
• Epitope mapping of recombinant hepatitis B surface antigen by murine monoclonal antibodies.
• Establishment of human heterohybridoma and lymphoblastoid cell lines specific for the Rh D and C antigens.
• Expression profile of Wnt molecules in leukemic cells from Iranian patients with acute myeloblastic leukemia.
• Expression profile of orphan receptor tyrosine kinase (ROR1) and Wilms' tumor gene 1 (WT1) in different subsets of B-cell acute lymphoblastic leukemia.
• Fc receptor-like 1-5 molecules are similarly expressed in progressive and indolent clinical subtypes of B-cell chronic lymphocytic leukemia.
• HLA-DRB1, DQA1 and DQB1 Alleles and Haplotypes Frequencies in Iranian Healthy Adult Responders and Non-Responders to Recombinant Hepatitis B Vaccine.
• Immunogenicity of a triple diphtheria-tetanus-whole cell pertussis vaccine in Iranian preschool children.
• Immunophenotypic Characterization of the Leukemic B-cells from Iranian Patients with Chronic Lymphocytic Leukemia: Association between CD38 Expression and Disease Progression.
• Longitudinal determination of hepatitis B surface antigen-specific B lymphocyte frequency in healthy high responder adults after booster vaccination.
• Low dose revaccination induces robust protective anti-HBs antibody response in the majority of healthy non-responder neonates.