Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression.

Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression. Research Abstract Details 

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Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression. Abstract Text:

Ingrid B Renes,Melissa Verburg, Van Nispen,Jan A J M Taminiau,Hans A ,Jan Dekker,Alexandra W C Einerhand,

BACKGROUND AND AIMS: To gain insight in intestinal epithelial proliferation, cell death, and gene expression during experimental colitis rats were treated with dextran sulfate sodium (DSS) for 7 days. MATERIALS AND METHODS: Proximal and distal colonic segments were excised on days 2, 5, 7, and 28. Epithelial proliferation, cell death, enterocyte gene expression (carbonic anhydrase I (CA I) and goblet cell gene expression (mucin, MUC2; trefoil factor 3, TFF3) were studied immunohistochemically and biochemically. RESULTS: Proliferative activity was decreased in the proximal and distal colon at the onset of disease (day 2). However, during active disease (days 5-7) epithelial proliferation was increased in the entire proximal colon and in the proximity of ulcerations in the distal colon. During DSS treatment the number of apoptotic cells in the epithelium of both colonic segments was increased. In the entire colon surface enterocytes became flattened and CA I negative during active disease (day 5-7). Additionally, CA I levels in the distal colon significantly decreased during this phase. In contrast, during the regenerative phase (day 28) CA I levels were restored in the distal colon and up-regulated in the proximal colon. During all disease phases increased numbers of goblet cells were observed in the surface epithelium of the entire colon. In the distal colon TFF3 expression extended to the bottom of the crypts during active disease. Finally, MUC2 and TFF3 expression was increased in the proximal colon during disease. CONCLUSION: DSS affected the epithelium by inhibiting proliferation and inducing apoptosis. DSS-induced inhibition of CA I expression indicates down-regulation of specific enterocyte functions. Accumulation of goblet cells in the surface epithelium and up-regulation of MUC2 and TFF3 expression in the proximal colon underline the importance of goblet cells in epithelial protection and repair, respectively.

Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression. Publishing Authors By Initials

IB Renes,M Verburg,DJ Van Nispen,JA Taminiau,HA ,J Dekker,AW Einerhand,

For similar pathological conditions, signs and symptoms: signs and symptoms: body weight: body weight changes: weight loss research abstracts see: pathological conditions, signs and symptoms: signs and symptoms: body weight: body weight changes: weight loss research

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Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: International journal of colorectal disease

VOLUME: 17

Page Numbers: 317-26

Journal Abbreviation:

ISSN: 0179-1958

DAY: 15

MONTH: 06

YEAR: 2002

Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression. Information

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LANGUAGE: eng

NlmUniqueID: 8607899

Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression. Keywords Mesh Terms:

KEYWORDS: Weight Loss

MESH TERMS: drug effects

Chemical & Substance for Abstract: Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression. Information

Substance Name: Carbonic Anhydrase I

Registry Number: EC 4.2.1.-

Grant and Affiliation Information for Epithelial proliferation, cell death, and gene expression in experimental colitis: alterations in carbonic anhydrase I, mucin MUC2, and trefoil factor 3 expression.

AFFILIATION: Department of Pediatrics, Erasmus Medical Center, Dr Molewaterplein 50, and Sophia Children's Hospital, 3015 GE Rotterdam, The Netherlands.

Country: Germany

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MEDLINETA: Int J Colorectal Dis

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