Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors.

Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors. Abstract Text:

Hans T Bjornsson,Lindsey J Brown,M Danielle Fallin,Michael A Rongione,Marina Bibikova,Eliza Wickham,Jian-Bing Fan,Andrew P Feinberg,

Loss of imprinting (LOI) of the IGF2 gene (which encodes insulin-like growth factor II) is the most common genetic or epigenetic alteration in Wilms tumor; LOI involves aberrant activation of the normally repressed maternally inherited allele. We found previously that LOI of IGF2 occurs in approximately half of all Wilms tumors (i.e., those arising from lineage-committed nephrogenic progenitor cells). We investigated whether LOI of IGF2 is associated with relaxation of imprinting at loci other than IGF2 or with widespread alterations in DNA methylation. We stratified 59 Wilms tumor samples by IGF2 LOI status by use of hot-stop reverse transcription-polymerase chain reaction and/or methylation analysis of the differentially methylated region of the H19 gene and identified 31 samples with and 28 without LOI. We used quantitative allele-specific expression analysis to determine whether six other imprinted genes (i.e., H19, KCNQ1, LIT1, TSSC5, GRB10, and MEG3) had subtle LOI. No statistically significant difference in allele-specific expression between Wilms tumor with or without LOI was found for LIT1, TSSC5, GRB10, and MEG3. For the KCNQ1 gene there was a slight difference between the groups with (37.0%, 95% confidence interval [CI] = 31.8% to 42.2%) and without (27.7%, 95% CI = 21.8% to 33.5%) LOI (P = .02 for F test of group differences in a mixed-effects model). For H19, we also found a slight difference between the groups with (7.5%, 95% CI = 2.4% to 12.7%) and without (2.2%, 95% CI = -3.2% to 7.6%) LOI of IGF2 (P = .15 for F test). In 27 tumor samples, we also used a microarray technique to analyze methylation of 378 genes, 38 of which were suspected or confirmed imprinted genes. We found that statistically significant alterations in only the differentially methylated region of the H19 gene were associated with LOI of IGF2. Thus, epigenetic alterations in Wilms tumors are not widespread, supporting the gene and lineage specificity of LOI of IGF2.

Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors. Publishing Authors By Initials

HT Bjornsson,LJ Brown,MD Fallin,MA Rongione,M Bibikova,E Wickham,JB Fan,AP Feinberg,

For similar neoplasms: neoplasms by histologic type: neoplasms, complex and mixed: wilms tumor research abstracts see: neoplasms: neoplasms by histologic type: neoplasms, complex and mixed: wilms tumor research

PUBMED ID PMID:

MEDLINE DATE:

Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of the National Cancer Institute

VOLUME: 99

Page Numbers: 1270-3

Journal Abbreviation: J. Natl. Cancer Inst.

ISSN: 1460-2105

DAY: 8

MONTH: 08

YEAR: 2007

Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503089

Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors. Keywords Mesh Terms:

KEYWORDS: Wilms Tumor

MESH TERMS: genetics

Chemical & Substance for Abstract: Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors. Information

Substance Name: Insulin-Like Growth Factor II

Registry Number: 67763-97-7

Grant and Affiliation Information for Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors.

AFFILIATION: Center for Epigenetics, Institute of Basic Biomedical Sciences, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Country: United States

AGENCY: United States NCI

GRANT: CA65145

ACRONYM: CA

MEDLINETA: J Natl Cancer Inst

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors Related Publications

• A case of diphtheria presenting some unusual features.
• A study of some aspects of peripheral visual acuity.
• A study of some aspects of peripheral visual acuity.
• Abuse of Prescription and Over-the-Counter Medications.
• An epigenetic approach to cancer etiology.
• Asthma; present status of therapy.
• Chemical studies on experimental hepatic congestion in the dog.
• Computed tomography-based tissue-engineered scaffolds in craniomaxillofacial surgery.
• DNA methylation signatures within the human brain.
• Depletion of CD8+ cells in sooty mangabey monkeys naturally infected with simian immunodeficiency virus reveals limited role for immune control of virus replication in a natural host species.
• Dissociating averageness and attractiveness: Attractive faces are not always average.
• Enhanced sensitivity to IGF-II signaling links loss of imprinting of IGF2 to increased cell proliferation and tumor risk.
• Epigenetic specificity of loss of imprinting of the IGF2 gene in Wilms tumors.
• Estimating the effectiveness of simian immunodeficiency virus-specific CD8+ T cells from the dynamics of viral immune escape.
• Histamine antagonists; an experimental and clinical study of N,N-dimethyl-N'-2-thiazolyl-N'-p-methoxybenzyl-ethylenediamine hydrochloride (194-B).
• Increase of photosensitivity in Ge-doped fibers under strain.
• Isolation of human oral keratinocyte progenitor/stem cells.
• Kinetics of NREM delta EEG power density across NREM periods depend on age and on delta-band designation.
• Multiple equilibria in complex chemical reaction networks: extensions to entrapped species models.
• Photochemical reaction of hydrogen with germanosilicate glass initiated by 3.4 5.4-eV ultraviolet light.
• Skin and oral mucosal substitutes.
• Sleep EEG evidence of sex differences in adolescent brain maturation.
• Substance-use outcomes at 18 months past baseline: the PROSPER Community-University Partnership Trial.
• The "hard problem" of anosognosia: delusional confabulation and anosognosia.
• The "nose drop nose" due to oxymetazoline (Afrin) and other topical vasoconstrictors.
• The increase in longitudinally measured sleepiness across adolescence is related to the maturational decline in low-frequency EEG power.
• The temporalis muscle flap in contemporary oral and maxillofacial surgery.
• Tipranavir: a new option for the treatment of drug-resistant HIV infection.
• Using correlational analyses to improve prevention strategies based on survey data from youth.
• Waking brain states and homeostatic requirement. Commentary on Franken P. The quality of waking and process S. Sleep 2007;30:126-7.