Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis.

Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis. Research Abstract Details 

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Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis. Abstract Text:

Joanne L Attema,Peter Papathanasiou,E Camilla Forsberg,Jian Xu,Stephen T Smale,Irving L Weissman,

Hematopoietic stem cells (HSC) produce all blood cell lineages by virtue of their capacity to self-renew and differentiate into progenitors with decreasing cellular potential. Recent studies suggest that epigenetic mechanisms play an important role in controlling stem cell potency and cell fate decisions. To investigate this hypothesis in HSC, we have modified the conventional chromatin immunoprecipitation assay allowing for the analysis of 50,000 prospectively purified stem and progenitor cells. Together with bisulfite sequencing analysis, we found that methylated H3K4 and AcH3 and unmethylated CpG dinucleotides colocalize across defined regulatory regions of lineage-affiliated genes in HSC. These active epigenetic histone modifications either accumulated or were replaced by increased DNA methylation and H3K27 trimethylation in committed progenitors consistent with gene expression. We also observed bivalent histone modifications at a lymphoid-affiliated gene in HSC and downstream transit-amplifying progenitors. Together, these data support a model in which epigenetic modifications serve as an important mechanism to control HSC multipotency.

Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis. Publishing Authors By Initials

JL Attema,P Papathanasiou,EC Forsberg,J Xu,ST Smale,IL Weissman,

For similar inorganic chemicals: electrolytes: ions: anions: sulfites research abstracts see: inorganic chemicals: electrolytes: ions: anions: sulfites research

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Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 104

Page Numbers: 12371-6

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 18

MONTH: 07

YEAR: 2007

Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis. Information

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LANGUAGE: eng

NlmUniqueID: 7505876

Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis. Keywords Mesh Terms:

KEYWORDS: Sulfites

MESH TERMS: analysis

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Substance Name: Sulfites

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Grant and Affiliation Information for Epigenetic characterization of hematopoietic stem cell differentiation using miniChIP and bisulfite sequencing analysis.

AFFILIATION: Institute of Stem Cell Biology and Regenerative Medicine, Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. joattema@stanford.edu

Country: United States

AGENCY: United States NIDDK

GRANT: R01DK43726

ACRONYM: DK

MEDLINETA: Proc Natl Acad Sci U S A

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