Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells.

Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. Abstract Text:

Masakatsu Morita,Hidenori Matsuzaki,Toshiyoshi Yamamoto,Yasuo Fukami,Ushio Kikkawa,

Protein kinase C (PKC) delta is phosphorylated at Tyr311 and Tyr332 and its catalytic activity is enhanced in the H(2)O(2)-stimulated cells, but the enzymes that recognize these tyrosine residues, especially Tyr332, have been remained to be clarified. The analysis of the endogenous proteins in COS-7 cells revealed that PKCdelta binds to p66Shc, an adaptor protein containing two phosphotyrosine-binding domains, in a manner dependent on its tyrosine phosphorylation upon H(2)O(2) stimulation. The studies using the mutated PKCdelta clarified that PKCdelta associates with p66Shc through the phosphorylated Tyr332 residue. Epidermal growth factor (EGF) receptor was detected in the anti-p66Shc immunoprecipitate prepared from the H(2)O(2)-stimulated cells, and this receptor-type tyrosine kinase phosphorylated PKCdelta at Tyr332 in vitro. PKCdelta was, however, not tyrosine phosphorylated in the EGF-stimulated cells, whereas H(2)O(2)-induced tyrosine phosphorylation of PKCdelta and its association with p66Shc were strongly suppressed by EGF receptor kinase inhibitors such as AG1478 and PD153035. These results indicate that EGF receptor phosphorylates PKCdelta at Tyr332 in the H(2)O(2)-stimulated but not in the growth-factor treated cells, and suggest that PKCdelta in the complex with p66Shc and EGF receptor may play a role in the stress-signalling pathway.

Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. Publishing Authors By Initials

M Morita,H Matsuzaki,T Yamamoto,Y Fukami,U Kikkawa,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 143

Page Numbers: 31-8

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 23

MONTH: 10

YEAR: 2007

Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells.

AFFILIATION: Biosignal Research Center; and Research Center for Environmental Genomics, Kobe University, Kobe 657-8501, Japan.

Country: Japan

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells Related Publications

• Activation of protein kinase B induced by H(2)O(2) and heat shock through distinct mechanisms dependent and independent of phosphatidylinositol 3-kinase.
• Angiotensin II mimics the hypoxic effect on regulating trophoblast proliferation and differentiation in human placental explant cultures.
• Carbon beam therapy in recurrent ovarian cancer.
• Catalytic properties of yeast protein kinase C: difference between the yeast and mammalian enzymes.
• Distinct effects of saturated fatty acids on protein kinase C subspecies.
• Distribution of Endothelin-converting Enzyme-1 and Neutral Endopeptidase in Human Endometrium.
• EEG Analysis in Wakefulness Maintenance State against Sleepiness by Instantaneous Equivalent Bandwidths.
• Epidermal Growth Factor Receptor Phosphorylates Protein Kinase C {delta} at Tyr332 to form a Trimeric Complex with p66Shc in the H2O2-stimulated Cells.
• Expression of N-acetylglucosaminyltransferase V in endometrial cancer correlates with poor prognosis.
• Expression of Rho-family GTPases (Rac, cdc42, RhoA) and their association with p-21 activated kinase in adult rat peripheral nerve.
• Genetically identified complete hydatidiform mole coexisting with a live twin fetus: comparison with conventional diagnosis.
• High glucose-induced abnormal epidermal growth factor signaling.
• Involvement of SDF-1alpha/CXCR4 axis in the enhanced peritoneal metastasis of epithelial ovarian carcinoma.
• Irradiation-induced epithelial-mesenchymal transition (EMT) related to invasive potential in endometrial carcinoma cells.
• Opposite regulation of tyrosine-phosphorylation of p130(Cas) by insulin and insulin-like growth factor I.
• PTEN and Akt expression during growth of human ovarian follicles.
• Placenta previa increta/percreta in Japan: a retrospective study of ultrasound findings, management and clinical course.
• Possible involvement of SDF-1alpha/CXCR4-DPPIV axis in TGF-beta1-induced enhancement of migratory potential in human peritoneal mesothelial cells.
• Regulation of intracellular localization and transcriptional activity of FOXO4 by protein kinase B through phosphorylation at the motif sites conserved among the FOXO family.
• Ruby-Helix: an implementation of helical image processing based on object-oriented scripting language.
• THE INTRACELLULAR POTENTIAL OF THE CORNEAL EPITHELIUM.
• Three-dimensional structure of cytoplasmic dynein bound to microtubules.
• Three-dimensional structures of the flagellar dynein-microtubule complex by cryoelectron microscopy.
• Twist expression in patients with cervical cancer is associated with poor disease outcome.
• Uterine smooth muscle cells increase invasive ability of endometrial carcinoma cells through tumor-stromal interaction.
• [A case of ascending colon carcinoma metastasized to an inguinal hernia sac]
• [A case of metastatic rectal cancer from serous adenocarcinoma of the ovary.]
• [A clinical significance of peritoneal carcinomatosis to recurrent colorectal cancer with ileus]
• [Corneal potential as influenced by the contact lens]
• [Three successful cases with CPT-11 + CDDP chemotherapy where S-1 failed to respond to recurrent gastric cancer]