Epidermal growth factor inhibits Fas-mediated apoptosis in salivary epithelial cells of patients with primary Sjögren's syndrome.

Epidermal growth factor inhibits Fas-mediated apoptosis in salivary epithelial cells of patients with primary Sjögren's syndrome. Research Abstract Details 

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Epidermal growth factor inhibits Fas-mediated apoptosis in salivary epithelial cells of patients with primary Sjögren's syndrome. Abstract Text:

H Nakamura,A Kawakami,H Ida,T Koji,K Eguchi,H Nakamura,A Kawakami,H Ida,T Koji,K Eguchi,

OBJECTIVES: Cell death is detected in the ducts of labial salivary glands (LSG) of patients with primary Sjögren's syndrome (pSS). However, the counter-mechanism to inhibit the apoptotic process remains unclear. In this study, we studied the ability of epidermal growth factor (EGF) to activate the PI3K-Akt pathway and NF-kB in primary cultured salivary gland epithelial cells (SGEC) of pSS patients.METHODS:SGEC, obtained from 2 female pSS patients, were cultured and used for Hoechst staining and deoxynucleotidyltransferase-mediated dUTP nick end-labeling (TUNEL) assay. The frequency of apoptosis, detected by Hoechst staining, was quantified, and statistical significance was determined through unpaired student's t-test.RESULTS:Following twelve hours of stimulation, both PI3K inhibitors and anti-Fas antibody failed to induce apoptosis in primary cultured SGEC. However, the combination of anti-Fas antibody, along with LY294002 or Bay 11-7082, induced apoptosis which was statistically more significant than apoptosis found in the control cells (p < 0.01). Interestingly, the apoptosis induced by anti-Fas antibody along with LY294002 was clearly inhibited by the addition of 10 ng/ml EGF. Furthermore, the results of the TUNEL assay clearly indicated apoptosis through stimulation with anti-Fas antibody and LY294002 or Bay 11-7082. Furthermore, the apoptosis was completely blocked by the addition of EGF.CONCLUSION:: Our results suggest that salivary epithelial cells are protected from Fas mediated apoptosis, through cell survival factors including either the PI3K-Akt pathway or NF-kB.

Epidermal growth factor inhibits Fas-mediated apoptosis in salivary epithelial cells of patients with primary Sjögren's syndrome. Publishing Authors By Initials

H Nakamura,A Kawakami,H Ida,T Koji,K Eguchi,H Nakamura,A Kawakami,H Ida,T Koji,K Eguchi,

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MEDLINE DATE: 2007 Nov-Dec

Epidermal growth factor inhibits Fas-mediated apoptosis in salivary epithelial cells of patients with primary Sjögren's syndrome. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Clinical and experimental rheumatology

VOLUME: 25

Page Numbers: 831-7

Journal Abbreviation: Clin. Exp. Rheumatol.

ISSN: 0392-856X

DAY: 4

MONTH: 01

YEAR: 2008

Epidermal growth factor inhibits Fas-mediated apoptosis in salivary epithelial cells of patients with primary Sjögren's syndrome. Information

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LANGUAGE: eng

NlmUniqueID: 8308521

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AFFILIATION: 1First Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.

Country: Italy

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MEDLINETA: Clin Exp Rheumatol

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