Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo.

Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo. Abstract Text:

Gloria S Kwon,Anna-Katerina Hadjantonakis,

Expression of T-box family member Eomesodermin (Tbr2) is spatiotemporally restricted in the mouse embryo; initially expressed in extraembryonic lineages in the sequential progression from the trophectoderm of the blastocyst, its derivatives the extraembryonic ectoderm, and thereafter the chorion, in addition to the visceral endoderm and primitive streak at gastrula stages, and the telencephalon at later stages. We describe the spatiotemporal expression of GFP in embryos of a Tg(Eomes::GFP) BAC transgenic strain, and have compared it with the localization of endogenous Eomes transcripts and protein. Our analysis reveals the following: (1) robust easily visualized reporter expression in live hemizygous transgenic embryos, (2) increased levels of expression in live homozygous transgenic embryos that are compatible with embryo viability, and (3) a close correlation between endogenous Eomes and GFP reporter expression in BAC transgenic embryos. These features establish the Tg(Eomes::GFP) BAC transgenic strain as a novel reagent for both live imaging and the isolation of Eomes expressing cells from specific locations within the embryo.

Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo. Publishing Authors By Initials

GS Kwon,AK Hadjantonakis,

For similar cells: trophoblasts research abstracts see: cells: trophoblasts research

PUBMED ID PMID:

MEDLINE DATE:

Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genesis (New York, N.Y. : 2000)

VOLUME: 45

Page Numbers: 208-17

Journal Abbreviation:

ISSN: 1526-954X

DAY: 3

MONTH: Apr

YEAR: 2007

Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100931242

Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo. Keywords Mesh Terms:

KEYWORDS: Trophoblasts

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo.

AFFILIATION: Developmental Biology Program, Sloan-Kettering Institute, New York, New York 10021, USA.

Country: United States

AGENCY: United States NICHD

GRANT: R01-HD052115

ACRONYM: HD

MEDLINETA: Genesis

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo Related Publications

• A cornucopia of delights for the mouse fancier.
• Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst.
• Eomes::GFP-a tool for live imaging cells of the trophoblast, primitive streak, and telencephalon in the mouse embryo.
• The endoderm of the mouse embryo arises by dynamic widespread intercalation of embryonic and extraembryonic lineages.
• The multidimensionality of cell behaviors underlying morphogenesis: a case study in ascidians.