Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products.

Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. Abstract Text:

Masayuki Wada,Cynthia J DeLong,Yu H Hong,Caroline J Rieke,Inseok Song,Ranjinder S Sidhu,Chong Yuan,Mark Warnock,Alvin H Schmaier,Chieko Yokoyama,Emer M Smyth,Stephen J Wilson,Garret A FitzGerald,R Michael Garavito,De Xin Sui,John W Regan,William L Smith,

Dietary fish oil containing omega 3 highly unsaturated fatty acids has cardioprotective and anti-inflammatory effects. Prostaglandins (PGs) and thromboxanes are produced in vivo both from the omega 6 fatty acid arachidonic acid (AA) and the omega 3 fatty acid eicosapentaenoic acid (EPA). Certain beneficial effects of fish oil may result from altered PG metabolism resulting from increases in the EPA/AA ratios of precursor phospholipids. Here we report in vitro specificities of prostanoid enzymes and receptors toward EPA-derived, 3-series versus AA-derived, 2-series prostanoid substrates and products. The largest difference was seen with PG endoperoxide H synthase (PGHS)-1. Under optimal conditions purified PGHS-1 oxygenates EPA with only 10% of the efficiency of AA, and EPA significantly inhibits AA oxygenation by PGHS-1. Two- to 3-fold higher activities or potencies with 2-series versus 3-series compounds were observed with PGHS-2, PGD synthases, microsomal PGE synthase-1 and EP1, EP2, EP3, and FP receptors. Our most surprising observation was that AA oxygenation by PGHS-2 is only modestly inhibited by EPA (i.e. PGHS-2 exhibits a marked preference for AA when EPA and AA are tested together). Also unexpectedly, TxA(3) is about equipotent to TxA(2) at the TP alpha receptor. Our biochemical data predict that increasing phospholipid EPA/AA ratios in cells would dampen prostanoid signaling with the largest effects being on PGHS-1 pathways involving PGD, PGE, and PGF. Production of 2-series prostanoids from AA by PGHS-2 would be expected to decrease in proportion to the compensatory decrease in the AA content of phospholipids that would result from increased incorporation of omega 3 fatty acids such as EPA.

Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. Publishing Authors By Initials

M Wada,CJ DeLong,YH Hong,CJ Rieke,I Song,RS Sidhu,C Yuan,M Warnock,AH Schmaier,C Yokoyama,EM Smyth,SJ Wilson,GA FitzGerald,RM Garavito,de X Sui,JW Regan,WL Smith,

For similar thromboxanes research abstracts see: thromboxanes research

PUBMED ID PMID:

MEDLINE DATE:

Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 22254-66

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 22

MONTH: 05

YEAR: 2007

Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. Keywords Mesh Terms:

KEYWORDS: Thromboxanes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products. Information

Substance Name: thromboxane A3

Registry Number: 60114-68-3

Grant and Affiliation Information for Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products.

AFFILIATION: Department of Biological Chemistry, University of Michigan, Ann Arbor, Michigan 48109, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL56773

ACRONYM: HL

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Enzymes and receptors of prostaglandin pathways with arachidonic acid-derived versus eicosapentaenoic acid-derived substrates and products Related Publications

• 10 do's and don'ts about tax homes.
• A gravitational lens.
• An animal evaluation of a paste of chitosan glutamate and hydroxyapatite as a synthetic bone graft material.
• An electrostrictive drive for fine pitch control in double-crystal monochromators.
• Animal model of human disease: inherited erythrocyte glutathione deficiency.
• Bacterial pathogens of the respiratory tract of the newborn infant.
• Brain uptake of nonsteroidal anti-inflammatory drugs: ibuprofen, flurbiprofen, and indomethacin.
• Calculating Society's Willingness to Pay for a QALY : Key Questions for Discussion.
• Characterizing the structures and folding of free proteins using 2-D gas-phase separations: observation of multiple unfolded conformers.
• Coccidioidomycosis.
• Demoralization, anhedonia and grief in patients with severe physical illness.
• Developmental outcomes after early or delayed insertion of tympanostomy tubes.
• Effects of hormone-induced superovulation on latent infectious bovine rhinotracheitis virus in cattle.
• Euphemistic codes and tell-tale hearts: humane assistance in end-of-life cases.
• Evaluation of beta-endorphin and naloxone on bovine uterine motility.
• Gastrointestinal disease in adolescents.
• Increased amyloid- levels in APPSWE transgenic mice treated chronically with a physiological high-fat high-cholesterol diet.
• Joys of teaching nursing.
• Occupational health in India.
• Odontoblast morphology and dental repair.
• Practical management of tuberculosis.
• Preventing and treating: Heart disease in women.
• Synthesis of 4,4-difluoroglycosides using ring-closing metathesis.
• The Herpesvirus capsid surface protein, VP26, and the majority of the tegument proteins are dispensable for capsid transport toward the nucleus.
• The effects of rhBMP-2 on human osteosarcoma cells and human gingival fibroblasts in vitro.
• The effects of xylazine hydrochloride on intrauterine pressure in the cow.
• The reliability of willingness to pay for changes in health status.
• The role of primary care in infertility management.
• What is happening in the real world in lipid therapy and is it appropriate? The need for a paradigm shift.
• X-ray Imaging and Microspectroscopy of Plants and Fungi.