Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA.

Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA. Research Abstract Details 

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Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA. Abstract Text:

Lincoln G Scott,Bernhard H Geierstanger,James R Williamson,Mirko Hennig,

The production of isotopically labeled RNA remains critical to current NMR structural studies. One approach to obtain simple NMR spectra is to label with a nucleus that is not naturally occurring in RNA. Fluorine-19 can serve as a sensitive site-specific probe upon incorporation into RNA. Here we report the efficient in vitro enzymatic synthesis of 2-fluoroadenosine-5'-triphosphate and its incorporation into the HIV-2 transactivation region (TAR) of RNA by DNA template-directed transcription using phage T7 RNA polymerase. We provide unequivocal evidence for this 19F-substituted base analogue capability to selectively interact with uracil, forming 2F-A-U base pairs in RNA. The introduction of a 2-fluoroadenyl substitution is relatively nonperturbing and provides us with uniquely positioned, sensitive NMR reporter groups to monitor structural changes in the local RNA environment.

Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA. Publishing Authors By Initials

LG Scott,BH Geierstanger,JR Williamson,M Hennig,

For similar natural sciences: physics: thermodynamics research abstracts see: natural sciences: physics: thermodynamics research

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Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 126

Page Numbers: 11776-7

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 29

MONTH: Sep

YEAR: 2004

Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA. Information

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LANGUAGE: eng

NlmUniqueID: 7503056

Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA. Keywords Mesh Terms:

KEYWORDS: Thermodynamics

MESH TERMS: chemistry

Chemical & Substance for Abstract: Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA. Information

Substance Name: Adenylate Kinase

Registry Number: EC 2.7.4.3

Grant and Affiliation Information for Enzymatic synthesis and 19F NMR studies of 2-fluoroadenine-substituted RNA.

AFFILIATION: Department of Molecular Biology, The Skaggs Institute for Chemical Biology, The Scripps Research Institute, 10550 North Torrey Pines Road, MB33, La Jolla, California 92037, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: GM 53757

ACRONYM: GM

MEDLINETA: J Am Chem Soc

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