Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells.

Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells. Research Abstract Details 

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Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells. Abstract Text:

T Kobayashi,K Honke,S Gasa,Y Saga,T Miyazaki,K Tadano-Aritomi,I Ishizuka,A Makita,

Biosynthesis of sulfoglycolipid is markedly increased in a human renal cancer cell line, SMKT-R3. We investigated the sulfotransferase catalyzing the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to gangliotriaosylceramide (Gg3Cer) in SMKT-R3 cells. On thin-layer chromatography, the reaction product comigrated with Gg3Cer III3-sulfate (SM2b), not with Gg3Cer II3-sulfate (SM2a). To examine which monosaccharide of Gg3Cer was sulfated, the product was treated with beta-hexosaminidase A. Unlike authentic SM2a, of which the non-reducing terminal N-acetylgalactosamine was cleaved off, the product was not hydrolyzed. These results suggest that sulfate was transferred to the non-reducing terminal N-acetylgalactosamine. Gg3Cer sulfotransferase activity was independent of divalent cations but stimulated by Fe2+, and the optimal pH was approximately 6.5. The apparent Km values were 102 microM for PAPS and 348 microM for Gg3Cer. Gg3Cer II3,III3-bis-sulfate (SB2) and a sulfotransferase activity synthesizing SB2 from SM2a were also detected in SMKT-R3 cells.

Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells. Publishing Authors By Initials

T Kobayashi,K Honke,S Gasa,Y Saga,T Miyazaki,K Tadano-Aritomi,I Ishizuka,A Makita,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: hexosaminidases: beta-n-acetylhexosaminidases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: hexosaminidases: beta-n-acetylhexosaminidases research

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Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 117

Page Numbers: 987-92

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 1995

Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells. Keywords Mesh Terms:

KEYWORDS: beta-N-Acetylhexosaminidases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells. Information

Substance Name: beta-N-Acetylhexosaminidases

Registry Number: EC 3.2.1.52

Grant and Affiliation Information for Enzymatic sulfation of gangliotriaosylceramide in human renal cancer cells.

AFFILIATION: Biochemistry Laboratory, Hokkaido University School of Medicine, Sapporo.

Country: JAPAN

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MEDLINETA: J Biochem

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