Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice.

Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice. Research Abstract Details 

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Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice. Abstract Text:

Wenling Chen,Guoxen Chen,Daphne L Head,David J Mangelsdorf,David W Russell,

Liver X receptors (LXRs) are nuclear receptors that play crucial roles in lipid metabolism in vivo and are activated by oxysterol ligands in vitro. The identity of the ligand that activates LXRs in vivo is uncertain. Here we provide two lines of evidence that oxysterols are LXR ligands in vitro and in vivo. First, overexpression of an oxysterol catabolic enzyme, cholesterol sulfotransferase, inactivates LXR signaling in several cultured mammalian cell lines but does not alter receptor response to the nonsterol agonist T0901317. Adenovirus-mediated expression of the enzyme in mice prevents dietary induction of hepatic LXR target genes by cholesterol but not by T0901317. Second, triple-knockout mice deficient in the biosynthesis of three oxysterol ligands of LXRs, 24S-hydroxycholesterol, 25-hydroxycholesterol, and 27-hydroxycholesterol, respond to dietary T0901317 by inducing LXR target genes in liver but show impaired responses to dietary cholesterol. We conclude that oxysterols are in vivo ligands for LXR.

Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice. Publishing Authors By Initials

W Chen,G Chen,DL Head,DJ Mangelsdorf,DW Russell,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

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Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cell metabolism

VOLUME: 5

Page Numbers: 73-9

Journal Abbreviation: Cell Metab.

ISSN: 1550-4131

DAY: 3

MONTH: Jan

YEAR: 2007

Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice. Information

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LANGUAGE: eng

NlmUniqueID: 101233170

Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: metabolism

Chemical & Substance for Abstract: Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice. Information

Substance Name: cholesterol sulfotransferase

Registry Number: EC 2.8.2.-

Grant and Affiliation Information for Enzymatic reduction of oxysterols impairs LXR signaling in cultured cells and the livers of mice.

AFFILIATION: Department of Molecular Genetics, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Country: United States

AGENCY: United States NIAMS

GRANT: AR51943

ACRONYM: AR

MEDLINETA: Cell Metab

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