Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays.

Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays. Research Abstract Details 

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Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays. Abstract Text:

Md Monsur Ali,Shunxing Su,Carlos D M Filipe,Robert Pelton,Yingfu Li,Md Monsur Ali,Shunxing Su,Carlos D M Filipe,Robert Pelton,Yingfu Li,

We demonstrate that DNA oligonucleotides covalently coupled to colloidal microgel can be manipulated by T4 DNA ligase for DNA ligation and by Phi29 DNA polymerase for rolling circle amplification (RCA). We also show that the long single-stranded RCA product can generate intensive fluorescence upon hybridization with complementary fluorescent DNA probe. We believe DNA-microgel conjugates can be explored for the development of DNA based bioassays and biosensors.

Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays. Publishing Authors By Initials

MM Ali,S Su,CD Filipe,R Pelton,Y Li,MM Ali,S Su,CD Filipe,R Pelton,Y Li,

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Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Chemical communications (Cambridge, England)

VOLUME:

Page Numbers: 4459-61

Journal Abbreviation: Chem. Commun. (Camb.)

ISSN: 1359-7345

DAY: 22

MONTH: 08

YEAR: 2007

Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays. Information

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LANGUAGE: eng

NlmUniqueID: 9610838

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Grant and Affiliation Information for Enzymatic manipulations of DNA oligonucleotides on microgel: towards development of DNA-microgel bioassays.

AFFILIATION: Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, ON, L8N 3Z5, Canada.

Country: England

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MEDLINETA: Chem Commun (Camb)

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