Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface.

Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface. Abstract Text:

Mary Anne Nelson,Efthimia Papastavros,Maud Dodlinger,David S Hage,

The use of antibodies in HPLC columns for on-line immunoextraction combined with reversed-phase liquid chromatography (RPLC) is of growing interest in environmental and agricultural analysis. This technique is typically performed by using a small RPLC precolumn to capture and concentrate analytes as they elute from the immunoextraction column; however, there is little information on the conditions required for optimizing this interface. This study examined the behavior of this interface by using 2,4-dichlorophenoxyacetic acid (2,4-D) and related herbicides as model analytes. It was found that analyte dissociation from immunoextraction columns followed first-order decay and that the elution of these analytes through the immunoextraction/RPLC interface gave an exponentially modified Gaussian profile. Computer simulations were used to see how analyte elution through the interface changed with different dissociation and retention conditions. Several guidelines were developed from this work that could be used for developing and optimizing on-line immunoextraction/RPLC systems for other chemicals of environmental or agricultural interest.

Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface. Publishing Authors By Initials

MA Nelson,E Papastavros,M Dodlinger,DS Hage,

For similar investigative techniques: immunologic techniques research abstracts see: investigative techniques: immunologic techniques research

PUBMED ID PMID:

MEDLINE DATE:

Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of agricultural and food chemistry

VOLUME: 55

Page Numbers: 3788-97

Journal Abbreviation: J. Agric. Food Chem.

ISSN: 0021-8561

DAY: 25

MONTH: 04

YEAR: 2007

Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374755

Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface. Keywords Mesh Terms:

KEYWORDS: Immunologic Techniques

MESH TERMS: instrumentation

Chemical & Substance for Abstract: Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface. Information

Substance Name: 2,4-Dichlorophenoxyacetic Acid

Registry Number: 94-75-7

Grant and Affiliation Information for Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface.

AFFILIATION: Chemistry Department, University of Nebraska-Lincoln, Lincoln, Nebraska 68588-0304, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM044931

ACRONYM: GM

MEDLINETA: J Agric Food Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface Related Publications

• A 24-year-old man with mediastinal mass, dyspnea, and a pleural effusion.
• Amine-handling properties of APUD-cells in the bronchial epithelium of human foetuses and in the epithelium of the main bronchi of human adults.
• An 81-year-old man with lactic acidosis, refractory hypoglycemia, and lymphocytosis.
• Analysis of free drug fractions using near-infrared fluorescent labels and an ultrafast immunoextraction/displacement assay.
• Analysis of free hormone fractions by an ultrafast immunoextraction/displacement immunoassay: studies using free thyroxine as a model system.
• Applications of silica supports in affinity chromatography.
• Characterization of glycation adducts on human serum albumin by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
• Corticosteroids for Blastomycosis-Induced ARDS: A Report of Two Patients and Review of the Literature.
• Development of an affinity silica monolith containing human serum albumin for chiral separations.
• Development of sulfhydryl-reactive silica for protein immobilization in high-performance affinity chromatography.
• Earlier citation: Barrel sling technique for ankle traction.
• Electron microscopic identification of endocrine cells in the bronchial epithelium of human foetuses.
• Endocrine cells in the bronchial mucosa of human foetuses.
• Endocrine cells in the bronchial mucosa of human foetuses.
• Environmental analysis by on-line immunoextraction and reversed-phase liquid chromatography: optimization of the immunoextraction/RPLC interface.
• Evaluation of a hydrazide-linked alpha1-acid glycoprotein chiral stationary phase: separation of R- and S-propranolol.
• Gastrin and enteroglucagon cells in human antra, with special reference to intestinal metaplasia.
• Heraklas on knots: sixteen surgical nooses and knots from the first century A.D.
• Histoplasmosis as a cause for a positive Fungitelltrade mark (1-->3)-beta-D-glucan test.
• How capsizing, flipping, and flyping of traditional knots can result in new endoscopic knots: a geometric review.
• Identification and quantitative studies of protein immobilization sites by stable isotope labeling and mass spectrometry.
• Immobilization of alpha(1)-acid glycoprotein for chromatographic studies of drug-protein binding II. correction for errors in association constant measurements.
• Influence of clay incorporation on the physical properties of polyethylene/Brazilian clay nanocomposites.
• Inversed, capsized, and flipped knots: unravelling a geometrical tangle.
• MMP-2 assessment as an indicator of wound healing: A feasibility study.
• On the origin and evolution of the roeder knot and loop-a geometrical review.
• Quantitative studies of allosteric effects by biointeraction chromatography: analysis of protein binding for low-solubility drugs.
• Surgical approach to the external and internal nose: with a supplementary report on two cases of nasal glioma.
• [Characterization of language disorders in children with lead poisoning.]
• [Soft tissue defects treated with perforator flaps]