Entrapping enzyme in a functionalized nanoporous support.

Entrapping enzyme in a functionalized nanoporous support. Research Abstract Details 

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Entrapping enzyme in a functionalized nanoporous support. Abstract Text:

Chenghong Lei,Yongsoon Shin,Jun Liu,Eric J Ackerman,

The enzyme organophosphorus hydrolase (OPH) was spontaneously entrapped in carboxylethyl- or aminopropyl-functionalized mesoporous silica with rigid, uniform open-pore geometry (30 nm). This approach yielded larger amounts of protein loading and much higher specific activity of the enzyme when compared to the unfunctionalized mesoporous silica and normal porous silica with the same pore size. When OPH was incubated with the functionalized mesoporous silica, protein molecules were sequestered in or excluded from the porous material, depending on electrostatic interaction with the charged functional groups. OPH entrapped in the organically functionalized nanopores showed an exceptional high immobilization efficiency of more than 200% and enhanced stability far exceeding that of the free enzyme in solution. The combination of high protein loading, high immobilization efficiency and stability is attributed to the large and uniform pore structure, and to the optimum environment introduced by the functional groups.

Entrapping enzyme in a functionalized nanoporous support. Publishing Authors By Initials

C Lei,Y Shin,J Liu,EJ Ackerman,

For similar inorganic chemicals: minerals: silicon dioxide research abstracts see: inorganic chemicals: minerals: silicon dioxide research

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Entrapping enzyme in a functionalized nanoporous support. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of the American Chemical Society

VOLUME: 124

Page Numbers: 11242-3

Journal Abbreviation: J. Am. Chem. Soc.

ISSN: 0002-7863

DAY: 25

MONTH: Sep

YEAR: 2002

Entrapping enzyme in a functionalized nanoporous support. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7503056

Entrapping enzyme in a functionalized nanoporous support. Keywords Mesh Terms:

KEYWORDS: Silicon Dioxide

MESH TERMS: chemistry

Chemical & Substance for Abstract: Entrapping enzyme in a functionalized nanoporous support. Information

Substance Name: Aryldialkylphosphatase

Registry Number: EC 3.1.8.1

Grant and Affiliation Information for Entrapping enzyme in a functionalized nanoporous support.

AFFILIATION: Pacific Northwest National Laboratory, Richland, Washington 99352, USA.

Country: United States

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MEDLINETA: J Am Chem Soc

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