Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat.

Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat. Abstract Text:

Stacy M Townsend,Edward Hurrell,Ignacio Gonzalez-Gomez,James Lowe,Jonathan G Frye,Stephen Forsythe,Julie L Badger,

Enterobacter sakazakii is an opportunistic pathogen associated with contaminated powdered infant formula and a rare cause of Gram-negative sepsis that can develop into meningitis and brain abscess formation in neonates. Bacterial pathogenesis remains to be fully elucidated. In this study, the host inflammatory response was evaluated following intracranial inoculation of Ent. sakazakii into infant rats. Infiltrating macrophages and neutrophils composed multiple inflammatory foci and contained phagocytosed bacteria. Several genotypically distinct Ent. sakazakii strains (16S cluster groups 1-4) were shown to invade rat capillary endothelial brain cells (rBCEC4) in vitro. Further, the persistence of Ent. sakazakii in macrophages varied between strains. The presence of putative sod genes and SOD activity may influence the survival of acidic conditions and macrophage oxidase and contribute to Ent. sakazakii intracellular persistence. The influence of macrophage uptake of Ent. sakazakii on immunoregulatory cytokine expression was assessed by ELISA. This demonstrated that the IL-10/IL-12 ratio is high after 24 h. This is suggestive of a type 2 immune response which is inefficient in fighting intracellular infections. These findings may help explain how the diversity in virulence traits among Ent. sakazakii isolates and an unsuccessful immune response contribute to the opportunistic nature of this infection.

Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat. Publishing Authors By Initials

SM Townsend,E Hurrell,I Gonzalez-Gomez,J Lowe,JG Frye,S Forsythe,JL Badger,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Microbiology (Reading, England)

VOLUME: 153

Page Numbers: 3538-47

Journal Abbreviation: Microbiology (Reading, Engl.)

ISSN: 1350-0872

DAY: 27

MONTH: Oct

YEAR: 2007

Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9430468

Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat.

AFFILIATION: School of Biomedical and Natural Sciences, Nottingham Trent University, Clifton Lane, Nottingham NG11 8NS, UK. stacy.townsend@hotmail.com

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Microbiology

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat Related Publications

• "If you want me to treat you like an adult, start acting like one!" Comparing the criteria that emerging adults and their parents have for adulthood.
• A nursing perspective of depression in cancer survivors and their partners.
• A semimicro diffusion method for the characterization of high-polymer fractions.
• Addition of osmium tetroxide to dinaphthylethylenes.
• Agouti signaling protein stimulates cell division in "viable yellow" (A(vy)/a) mouse liver.
• Alcohol dependence and simultaneous cocaine and alcohol use in cocaine-dependent patients.
• Computerized behavior therapy for opioid-dependent outpatients: a randomized controlled trial.
• Connective tissue fibroblast response to acupuncture: dose-dependent effect of bidirectional needle rotation.
• Critical care nurse intern program: addressing psychological reactions related to critical care nursing.
• Dietary soy protein isolate ameliorates atherosclerotic lesions in apolipoprotein e-deficient mice potentially by inhibiting monocyte chemoattractant protein-1 expression.
• Disseminated lupus erythematosus occurring among student nurses.
• Dose-dependent effects of alcohol on insulin signaling: partial explanation for biphasic alcohol impact on human health.
• Effects of pregnancy and nutritional status on alcohol metabolism.
• Effects of varying the monetary value of voucher-based incentives on abstinence achieved during and following treatment among cocaine-dependent outpatients.
• Elimination of water vapor in infra-red spectrometers.
• Emergency care practitioners (ECP): Practice and performance in the UK West country - A case study.
• Enterobacter sakazakii invades brain capillary endothelial cells, persists in human macrophages influencing cytokine secretion and induces severe brain pathology in the neonatal rat.
• Heat stress causes inhibition of the de novo synthesis of antenna proteins and photobleaching in cultured Symbiodinium.
• Laboratory Diagnosis of Endemic Typhus and Rocky Mountain Spotted Fever.
• Maternal obesity at conception programs obesity in the offspring.
• No difference indicated in electroencephalographic power spectral analysis in 3- and 6-month-old infants fed soy- or milk-based formula.
• Peroxisomal Malate Dehydrogenase Is Not Essential for Photorespiration in Arabidopsis But Its Absence Causes an Increase in the Stoichiometry of Photorespiratory CO2 Release.
• Regulation of the hepatic CYP 2E1 gene during chronic alcohol exposure: lack of an ethanol response element in the proximal 5'-flanking sequence.
• The Isolation Time of Scarlet Fever.
• Toxic level of fluorine in water supplies.