Enhancement of keratinocyte performance in the production of tissue-engineered skin using a low-calcium medium.

Enhancement of keratinocyte performance in the production of tissue-engineered skin using a low-calcium medium. Research Abstract Details 

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Enhancement of keratinocyte performance in the production of tissue-engineered skin using a low-calcium medium. Abstract Text:

Catherine A Hernon,Caroline A Harrison,Daniel J A Thornton,Sheila MacNeil,Catherine A Hernon,Caroline A Harrison,Daniel J A Thornton,Sheila MacNeil,

The success of laboratory-expanded autologous keratinocytes for the treatment of severe burn injuries is often compromised by their lack of dermal remnants and failure to establish a secure dermo-epidermal junction on the wound bed. We have developed a tissue-engineered skin substitute for in vivo use, based on a sterilized donor human dermis seeded with autologous keratinocytes and fibroblasts. However, culture rates are currently too slow for clinical use in acute burns. Our aim in this study was to increase the rate of production of tissue-engineered skin. Two approaches were explored: one using a commercial low-calcium media and the other supplementing well-established media for keratinocyte culture with the calcium-chelating agent ethylene glutamine tetra-acetic acid (EGTA). Using commercial low-calcium media for both the initial cell culture and subsequent culture of tissue-engineered skin did not produce tissue suitable for clinical use. However, it was possible to enhance the initial proliferation of keratinocytes and to increase their horizontal migration in tissue-engineered skin by supplementing established culture medium with 0.04 mM EGTA without sacrificing epidermal attachment and differentiation. Enhancement of keratinocyte migration with EGTA was also maximal in the absence of fibroblasts or basement membrane.

Enhancement of keratinocyte performance in the production of tissue-engineered skin using a low-calcium medium. Publishing Authors By Initials

CA Hernon,CA Harrison,DJ Thornton,S MacNeil,CA Hernon,CA Harrison,DJ Thornton,S MacNeil,

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Enhancement of keratinocyte performance in the production of tissue-engineered skin using a low-calcium medium. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Wound repair and regeneration : official publicati

VOLUME: 15

Page Numbers: 718-26

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ISSN: 1067-1927

DAY: 31

MONTH: 10

YEAR: 2007

Enhancement of keratinocyte performance in the production of tissue-engineered skin using a low-calcium medium. Information

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LANGUAGE: eng

NlmUniqueID: 9310939

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Grant and Affiliation Information for Enhancement of keratinocyte performance in the production of tissue-engineered skin using a low-calcium medium.

AFFILIATION: Tissue Engineering Group, Department of Engineering Materials and Division of Biomedical Sciences and Medicine, Kroto Research Institute, University of Sheffield North Campus, Sheffield, United Kingdom.

Country: United States

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MEDLINETA: Wound Repair Regen

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