Enhancement of dietary protein digestion by conjugated bile acids.

Enhancement of dietary protein digestion by conjugated bile acids. Research Abstract Details 

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Enhancement of dietary protein digestion by conjugated bile acids. Abstract Text:

Jonathan Gass,Harmit Vora,Alan F Hofmann,Gary M Gray,Chaitan Khosla,

BACKGROUND & AIMS: Conjugated bile acids promote absorption of dietary lipids by solubilizing them in mixed micelles. Bile acids are not considered to facilitate the digestion of other nutrients. METHODS: The effect of conjugated bile acids on the rate of protein hydrolysis by trypsin and chymotrypsin was examined in vitro. Common dietary proteins and 2 bacterial glutenases (proposed oral therapies for celiac sprue) were proteolyzed in the absence or presence of a 10 mmol/L conjugated bile acid mixture, simulating human bile composition. Lipolysis products (monoolein) and fatty acid were also evaluated to simulate postprandial intestinal contents. RESULTS: Conjugated bile acids dramatically enhanced the proteolysis of several dietary proteins, including beta-lactoglobulin, bovine serum albumin, myoglobin, and a commercially available dietary protein supplement. For beta-lactoglobulin, a cow's milk allergen that is resistant to pepsin cleavage, bile acids enhanced its proteolysis by pancreatic proteases even after incubation under gastric conditions. Exposure of prolyl endopeptidases to bile acids made them more susceptible to pancreatic proteases under simulated intestinal conditions. The conjugated bile acid effect was most pronounced in the presence of dihydroxy bile acids and was observable at bile concentrations below the critical micellar concentration but to a much greater extent at concentrations above the critical micellar concentration. CONCLUSIONS: We propose that, in addition to promoting lipid absorption, conjugated bile acids affect the digestion and assimilation of dietary proteins by accelerating hydrolysis by pancreatic proteases. These findings have implications for intraluminal protein breakdown and assimilation in the upper small intestine.

Enhancement of dietary protein digestion by conjugated bile acids. Publishing Authors By Initials

J Gass,H Vora,AF Hofmann,GM Gray,C Khosla,

For similar enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research abstracts see: enzymes and coenzymes: enzymes: hydrolases: peptide hydrolases: endopeptidases: serine endopeptidases: trypsin research

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Enhancement of dietary protein digestion by conjugated bile acids. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Gastroenterology

VOLUME: 133

Page Numbers: 16-23

Journal Abbreviation: Gastroenterology

ISSN: 0016-5085

DAY: 14

MONTH: 04

YEAR: 2007

Enhancement of dietary protein digestion by conjugated bile acids. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 374630

Enhancement of dietary protein digestion by conjugated bile acids. Keywords Mesh Terms:

KEYWORDS: Trypsin

MESH TERMS: pharmacology

Chemical & Substance for Abstract: Enhancement of dietary protein digestion by conjugated bile acids. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Enhancement of dietary protein digestion by conjugated bile acids.

AFFILIATION: Department of Chemical Engineering, Stanford University, Stanford, California, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK 64891

ACRONYM: DK

MEDLINETA: Gastroenterology

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