Enhanced growth of myelodysplastic colonies in hypoxic conditions.

Enhanced growth of myelodysplastic colonies in hypoxic conditions. Research Abstract Details 

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Enhanced growth of myelodysplastic colonies in hypoxic conditions. Abstract Text:

James Edwin Thompson,Joseph Patrick Conlon,Xiaowei Yang,Patricia Vanessa Sanchez,Martin Carroll,

OBJECTIVE: To determine the response of bone marrow progenitor cells from patients with myelodysplastic syndromes (MDS) to culture in physiologic oxygen tension. METHODS: Methylcellulose progenitor assays using both unfractionated bone marrow mononuclear cells (MNCs) and purified CD34(+) progenitors were performed in atmospheric oxygen (18.6% O(2)) or one of two levels of hypoxia (1% and 3% O(2)). Assays were performed using normal donor marrow, MDS patient marrow, acute myelogenous leukemia marrow or peripheral blood blasts, chronic phase chronic myelogenous leukemia (CML) marrow MNCs, and blast crisis CML peripheral blood. RESULTS: The majority of MDS samples showed decreased colony-forming units (CFU) in 18.6% O(2) compared to normal controls, as expected. However, in either 1% or 3% O(2), 9 of 13 MDS samples demonstrated augmentation of CFUs beyond that observed in normal controls, with 6 of 13 demonstrating a greater than ninefold augmentation. This effect is cell autonomous, as it persisted after purification of CD34(+) progenitor cells. Additionally, the augmented response to physiologic oxygen tension is specific to MDS, as it was not observed in either acute or chronic myelogenous leukemia samples. CONCLUSION: These results suggest that the reported decrease in MDS CFUs reflects greater sensitivity of MDS progenitors or their progeny to the nonphysiologic oxygen tensions routinely used in vitro, rather than a true decrease in progenitor frequency. Importantly, these experiments for the first time describe an experimental system that can be used to study the growth of primary cells from patients with MDS.

Enhanced growth of myelodysplastic colonies in hypoxic conditions. Publishing Authors By Initials

JE Thompson,JP Conlon,X Yang,PV Sanchez,M Carroll,

For similar inorganic chemicals: elements: chalcogens: oxygen research abstracts see: inorganic chemicals: elements: chalcogens: oxygen research

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Enhanced growth of myelodysplastic colonies in hypoxic conditions. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Experimental hematology

VOLUME: 35

Page Numbers: 21-31

Journal Abbreviation: Exp. Hematol.

ISSN: 0301-472X

DAY: 3

MONTH: Jan

YEAR: 2007

Enhanced growth of myelodysplastic colonies in hypoxic conditions. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 402313

Enhanced growth of myelodysplastic colonies in hypoxic conditions. Keywords Mesh Terms:

KEYWORDS: Oxygen

MESH TERMS: pathology

Chemical & Substance for Abstract: Enhanced growth of myelodysplastic colonies in hypoxic conditions. Information

Substance Name: Oxygen

Registry Number: 7782-44-7

Grant and Affiliation Information for Enhanced growth of myelodysplastic colonies in hypoxic conditions.

AFFILIATION: Division of Hematology and Oncology, University of Pennsylvania, Philadelphia, PA 19104-6160, USA. jamest@mail.med.upenn.edu

Country: Netherlands

AGENCY: United States NIDDK

GRANT: T32-DK07780

ACRONYM: DK

MEDLINETA: Exp Hematol

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