[Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines]

[Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines] Research Abstract Details 

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[Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines] Abstract Text:

Guo-yan Liu,Quan-xin Qu,Ruo-ran Mi,Jing Qi,Guo-yan Liu,Quan-xin Qu,Ruo-ran Mi,Jing Qi,

OBJECTIVE: To study changes of cisplatin sensitivity by RNA interfering the excision repair cross-complementing (ERCC) 1 gene in ovarian cancer cell lines. METHODS: The small interference RNA (siRNA) targeting ERCC1 gene was designed and synthesized by transcription in vitro, and transfected to ovarian cancer cell line ES-2. The mRNA and protein of ERCC1 were evaluated by means of RT-PCR, western blot and immunocytochemistry. The changes of cisplatin sensitivity after interference were examined by methyl thiazolyl tetrazolium (MTT) assay. RESULTS: In ES-2 cell, the mRNA and protein levels of ERCC1 were dramatically decreased 24, 48 and 72 hours after transfection. The sensitivity to cisplatin of ES-2 cell line was increased by 53.88 times after disturbing the ERCC1 gene. CONCLUSION: The sensitivity to cisplatin of ovarian cancer cell lines ES-2 could be enhanced by RNA interfering ERCC1 gene.

[Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines] Publishing Authors By Initials

GY Liu,QX Qu,RR Mi,J Qi,GY Liu,QX Qu,RR Mi,J Qi,

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[Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines] Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Zhonghua fu chan ke za zhi

VOLUME: 41

Page Numbers: 339-42

Journal Abbreviation: Zhonghua Fu Chan Ke Za Zhi

ISSN: 0529-567X

DAY: 9

MONTH: May

YEAR: 2006

[Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines] Information

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LANGUAGE: chi

NlmUniqueID: 16210370

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Grant and Affiliation Information for [Enhanced cisplatin cytotoxicity by RNA interfering the excision repair cross-complementing 1 gene in ovarian cancer cell lines]

AFFILIATION: Department of Obstetrics and Gynecology, General Hospital of Tianjin Medical University, Tianjin 300052, China.

Country: China

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MEDLINETA: Zhonghua Fu Chan Ke Za Zhi

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