Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures.

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Research Abstract Details 

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Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Abstract Text:

Hwa-Young Cho,Carolyn W T Lee-Parsons,Sung-Yong H Yoon,Hong Soon Rhee,Jong Moon Park,Hwa-Young Cho,Carolyn W T Lee-Parsons,Sung-Yong H Yoon,Hong Soon Rhee,Jong Moon Park,Hwa-Young Cho,Carolyn W T Lee-Parsons,Sung-Yong H Yoon,Hong Soon Rhee,Jong Moon Park,

Production of the benzophenanthridine alkaloids in Eschscholtzia californica suspension cell cultures was optimized by adding 0.5 mg methyl jasmonate (MJ) and 0.02 mg salicylic acid (SA)/g FCW after 7 days cultivation. Sanguinarine reached 24 mg/g DCW by such treatment; 10 times higher than in control cell cultures. MJ and SA induced expression of berberine bridge enzyme and 3'-hydroxy-(S)-N-methylcoclaurine-4'-O-methyltransferase, respectively. MJ plus SA induced over-expression of both enzymes.

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Publishing Authors By Initials

HY Cho,CW Lee-Parsons,SY Yoon,HS Rhee,JM Park,HY Cho,CW Lee-Parsons,SY Yoon,HS Rhee,JM Park,HY Cho,CW Lee-Parsons,SY Yoon,HS Rhee,JM Park,

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Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Biotechnology letters

VOLUME: 29

Page Numbers: 2001-5

Journal Abbreviation: Biotechnol. Lett.

ISSN: 0141-5492

DAY: 13

MONTH: 07

YEAR: 2007

Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Information

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LANGUAGE: eng

NlmUniqueID: 8008051

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Grant and Affiliation Information for Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures.

AFFILIATION: Advanced Environmental Biotechnology Research Center, School of Environmental Science and Engineering, Department of Chemical Engineering, Pohang University of Science and Technology, San 31, Hyoja-dong, Pohang, 790-784, South Korea, jmpark@postech.ac.kr.

Country: Netherlands

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MEDLINETA: Biotechnol Lett

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