Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor.

Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. Research Abstract Details 

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Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. Abstract Text:

S Brown,

Escherichia coli able to specifically adhere to iron oxide and not adhere to other metal oxides were constructed by genetic engineering. Concatamers of random oligonucleotides were introduced into a portion of a plasmid-borne lamB gene encoding an external domain of the phage lambda receptor. Bacteria able to adhere to iron oxide were selected by serial enrichment from the population of plasmid transformants. The concatameric nature of the inserted DNA allows a genetic analysis analogous to exons shuffling. Results of this genetic analysis indicate that in some isolates, part of the binding site is encoded by flanking vector sequences. This strategy may prove generally useful for identifying protein sequences able to recognize specific surfaces.

Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. Publishing Authors By Initials

S Brown,

For similar investigative techniques: genetic techniques: sequence alignment research abstracts see: investigative techniques: genetic techniques: sequence alignment research

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Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 89

Page Numbers: 8651-5

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 15

MONTH: Sep

YEAR: 1992

Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. Keywords Mesh Terms:

KEYWORDS: Sequence Alignment

MESH TERMS: metabolism

Chemical & Substance for Abstract: Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor. Information

Substance Name: ferric oxide

Registry Number: 1309-37-1

Grant and Affiliation Information for Engineered iron oxide-adhesion mutants of the Escherichia coli phage lambda receptor.

AFFILIATION: ABL-Basic Research Program, National Cancer Institute, Frederick Cancer Research and Development Center, MD 21702.

Country: UNITED STATES

AGENCY: United States NCRR

GRANT: S07-RR05761

ACRONYM: RR

MEDLINETA: Proc Natl Acad Sci U S A

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