Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase.

Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Research Abstract Details 

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Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Abstract Text:

Joseph A Covi,Steven C Hand,

We examine herein the contribution of V-ATPase activity to the energy budget of aerobically developing embryos of Artemia franciscana and discuss the results in the context of quiescence under anoxia. (31)P-NMR analysis indicates that intracellular pH and NTP levels are unaffected by acute incubation of dechorionated embryos with the V-ATPase inhibitor, bafilomycin A(1). Bafilomycin A(1) also has no significant effect on oxygen consumption by isolated mitochondria. Taken together, these data indicate that bafilomycin does not affect energy-producing pathways in the developing embryo. However, the V-ATPase inhibitor exhibits a concentration-dependent inhibition of oxygen consumption in aerobic embryos. A conservative analysis of respirometric data indicates that proton pumping by the V-ATPase, and processes immediately dependent on this activity, constitutes approximately 31% of the aerobic energy budget of the preemergent embryo. Given the complete absence of detectable Na(+)K(+)-ATPase activity during the first hours of aerobic development, it is plausible that the V-ATPase is performing a role in both the acidification of intracellular compartments and the energization of plasma membranes. Importantly, the high metabolic cost associated with maintaining these diverse proton gradients requires that V-ATPase activity be downregulated under anoxia in order to attain the almost complete metabolic depression observed in the quiescent embryo.

Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Publishing Authors By Initials

JA Covi,SC Hand,

For similar enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: adenosine triphosphatases: proton-translocating atpases: vacuolar proton-translocating atpases research abstracts see: enzymes and coenzymes: enzymes: hydrolases: acid anhydride hydrolases: adenosine triphosphatases: proton-translocating atpases: vacuolar proton-translocating atpases research

PUBMED ID PMID:

MEDLINE DATE: 2007 Jul-Aug

Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Physiological and biochemical zoology : PBZ

VOLUME: 80

Page Numbers: 422-32

Journal Abbreviation: Physiol. Biochem. Zool.

ISSN: 1522-2152

DAY: 4

MONTH: 05

YEAR: 2007

Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 100883369

Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Keywords Mesh Terms:

KEYWORDS: Vacuolar Proton-Translocating ATPases

MESH TERMS: metabolism

Chemical & Substance for Abstract: Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase. Information

Substance Name: Vacuolar Proton-Translocating ATPases

Registry Number: EC 3.6.1.-

Grant and Affiliation Information for Energizing an invertebrate embryo: bafilomycin-dependent respiration and the metabolic cost of proton pumping by the V-ATPase.

AFFILIATION: Division of Cellular, Developmental, and Integrative Biology, Department of Biological Science, Louisiana State University, Baton Rouge, Louisiana 70803, USA. jcovil@lsu.edu

Country: United States

AGENCY: United States NIGMS

GRANT: 1-R01-GM071345-01

ACRONYM: GM

MEDLINETA: Physiol Biochem Zool

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