Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats.

Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats. Research Abstract Details 

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Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats. Abstract Text:

Linah Al-Alem,Phillip J Bridges,Wen Su,Ming C Gong,Marc Iglarz,Chemyong Ko,

Proper function of the oviduct is critical to reproductive success with regulated contraction and relaxation facilitating transportation of the germ cells to the site of fertilization. Endothelin-2 (EDN2) is a potent vasoconstrictor produced by granulosa cells of the preovulatory follicle at the time of ovulation; however, whether this gonadotropin surge-induced peptide played a role in facilitating germ cell transportation by inducing oviductal contraction was unknown. The objectives of these experiments were (1) to determine whether the endothelin receptor system was present in the oviduct, (2) to test the hypothesis that EDN2 induces oviductal contraction via a specific endothelin receptor subtype, (3) to determine, as a possible alternate source of the ligand, whether mRNA for EDN2 was expressed in cumulus-oocyte complexes (COCs) within the oviduct, and (4) to determine whether EDN2 could overcome prostaglandin E(2) (PGE(2))-induced oviductal relaxation. Microarray and real-time PCR analysis indicated that mRNA for both the endothelin receptor subtypes (ET(A) and ET(B)) was present in the oviduct, whereas immunohistochemical examination revealed that ET(A) protein was the dominant isoform, present in the luminal epithelial cells of the oviduct. Real-time PCR analysis demonstrated that mRNA for EDN2 was expressed in COCs after ovulation. Isometric tension analysis indicated that EDN2 was a potent oviductal constrictor and that the contractile effect of EDN2 was mediated by the ET(A) and not the ET(B) receptor subtype. The oviductal contraction induced by EDN2 also reversed oviductal relaxation induced by PGE(2). In summary, ET(A) receptor-specific EDN2-induced contraction as a facilitator of oviductal function suggests a novel pathway involved in germ cell transport and hence mammalian fertility.

Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats. Publishing Authors By Initials

L Al-Alem,PJ Bridges,W Su,MC Gong,M Iglarz,C Ko,

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Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of endocrinology

VOLUME: 193

Page Numbers: 383-91

Journal Abbreviation: J. Endocrinol.

ISSN: 0022-0795

DAY: 3

MONTH: Jun

YEAR: 2007

Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375363

Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats. Keywords Mesh Terms:

KEYWORDS: Reverse Transcriptase Polymerase Chain R

MESH TERMS: metabolism

Chemical & Substance for Abstract: Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats. Information

Substance Name: Dinoprostone

Registry Number: 363-24-6

Grant and Affiliation Information for Endothelin-2 induces oviductal contraction via endothelin receptor subtype A in rats.

AFFILIATION: Division of Clinical and Reproductive Sciences, University of Kentucky, Lexington, Kentucky 40506, USA.

Country: England

AGENCY: United States NCRR

GRANT: P20 RR15592

ACRONYM: RR

MEDLINETA: J Endocrinol

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