Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt.

Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt. Abstract Text:

The phosphorylation of forkhead transcription factor FOXO3a by Akt is critical regulator of cell proliferation induced by serum. We show that endothelin-1 (ET-1) stimulation of primary human mesangial cells (HMCs) induces betaPix and p66Shc up-regulation, resulting in the formation of the betaPix/p66Shc complex. In transformed HMCs, ET-1 induces a biphasic phosphorylation of p66Shc and FOXO3a. The second phase leads to p27(kip1) down-regulation independently of Akt. Depletion of betaPix blocks the second phase of p66Shc and FOXO3a phosphorylation and prevents p27(kip1) down-regulation induced by ET-1. Depletion of either betaPix or p66Shc inhibits ET-1-induced cell proliferation. The expression of beta(1)Pix induces FOXO3a phosphorylation through activation of Rac1, ERK1/2, and p66Shc. Using either p66Shc- or Akt-depleted cells; we show that beta(1)Pix-induced FOXO3a phosphorylation requires p66Shc but not Akt. beta(1)Pix-induced p27(kip1) down-regulation was blocked by U0126 but not by wortmannin. Endogenous betaPix and FOXO3a are constitutively associated with endogenous p66Shc. FOXO3a and p66Shc binding requires beta(1)Pix homodimerization. Expression of beta(1)Pix homodimerization deficient mutant abrogates beta(1)Pix-induced p27(kip1) down-regulation and cell proliferation. Our results identify p66Shc and FOXO3a as novel partners of beta(1)Pix and represent the first direct evidence of beta(1)Pix in cell proliferation via Erk/p66Shc-dependent and Akt-independent mechanisms.

Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt. Publishing Authors By Initials

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Molecular biology of the cell

VOLUME: 19

Page Numbers: 2609-19

Journal Abbreviation: Mol. Biol. Cell

ISSN: 1059-1524

DAY: 2

MONTH: 04

YEAR: 2008

Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201390

Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt.

AFFILIATION: Kidney Disease Center, Department of Medicine, Division of Nephrology, Medical College of Wisconsin, Milwaukee, WI 53226.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Mol Biol Cell

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt Related Publications

• Caveolar endocytosis is critical for BK virus infection of human renal proximal tubular epithelial cells.
• Cranial nerve palsy in tetanus.
• Endothelin 1 induces beta 1Pix translocation and Cdc42 activation via protein kinase A-dependent pathway.
• Endothelin-1 Couples {beta}Pix to p66Shc: Role of {beta}Pix in Cell Proliferation through FOXO3a Phosphorylation and p27kip1 Down-Regulation Independently of Akt.
• Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity.
• Haloalkaliphilic diazotrophs in soda solonchak soils.
• Heterotrophic denitrification at extremely high salt and pH by haloalkaliphilic Gammaproteobacteria from hypersaline soda lakes.
• Intracellular trafficking pathway of BK Virus in human renal proximal tubular epithelial cells.
• Kinetics of luminescence and of electron transport in photosystem. II. When electron transport on acceptor side of this system is blocked.
• Methods to investigate the possibilities of using a three element periodic structure to suppress the transmission of energy in an elastic tube.
• Protein kinase A-dependent phosphorylation modulates beta1Pix guanine nucleotide exchange factor activity through 14-3-3beta binding.
• Repression of BK virus infection of human renal proximal tubular epithelial cells by pravastatin.
• The extracellular matrix of the spleen as a potential organizer of immune cell compartments.
• The genetically remote pathogenic strain NVH391-98 of the Bacillus cereus group is representative of a cluster of thermophilic strains.
• [Chemistry of hydrogen reduction of sulfates.]
• [Effect of acute stress on structuro-functional parameters of mouse liver and adrenal cortex]
• [Evaluation of the immune status in cattle herds with foot-and-mouth disease]
• [For the prevention of foot-and-mouth disease]
• [Investigation on certain aspects of Bacillus acetoethylicus using ferment poisons.]
• [Method of determination of the rate of running by fragments of the distance.]
• [Multiply achievements, eliminate deficiencies]
• [New methods of isolation of sulfate-reducing bacteria.]
• [On bacterial chemosynthesis in the Black Sea]
• [Prevention of noninfectious diseases among animals in Kaluga Province]
• [Primary transitional cell carcinoma of the prostate gland]
• [Skeletomuscular cell and its activity in the entire organism in relation to the effect of the nervous system.]
• [The antitoxic and pyrogenic properties of rabbit polymorphonuclear leukocytes]
• [The comparative effects of actinomycin D, imuren and hydrocortisone on interferon and antibody formation in mice]
• [The therapy of calves with toxic dyspepsia]
• [The treatment of dyspepsia in calves with titrated antibiotics and nitrofurans]