Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector.

Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector. Abstract Text:

Virna L Sales,Bret A Mettler,Marco Lopez-Ilasaca,John A Johnson,John E Mayer,

An unresolved question regarding tissue-engineered (TE) cardiac valves and vessels is the fate of the transplanted cells in vivo. We have developed a strategy to track the anatomic location of seeded cells within TE constructs and neighboring tissues using a retroviral vector system encoding green and red fluorescent proteins (GFPs and RFPs, respectively) in ovine circulating endothelial progenitor cells (EPCs) and bone marrow-derived mesenchymal stem cells (BMSCs). We demonstrate that stable transduction ex vivo with high-titer Moloney murine leukemia virus-based retroviral vector yields transduction efficiency of greater than 97% GFP(+) EPC- and RFP(+) mesenchymal stem cell (MSC)-derived cells. Cellular phenotype and transgene expression were also maintained through 25 subsequent passages. Using a retroviral vector system to distinguish our pre-seeded cells from tissue-resident progenitor cells and circulating endothelial and marrow-derived precursors, we simultaneously co-seeded 2 x 10(6) GFP(+) EPCs and 2 x 10(5) RFP(+) MSCs onto the TE patches. In a series of ovine pulmonary artery patch augmentation studies, transplanted GFP(+) EPC- and RFP(+) MSC-derived cells persisted within the TE patch 7 to 14 days after implantation, as identified using immunofluorescence. Analysis showed 81% luminal coverage of the TE patches before implantation with transduced cells, increasing to 96% at day 7 and decreasing to 67% at day 14 post-implantation. This suggests a temporal association between retroviral expression of progenitor cells and mediating effects of these cells on the physiological remodeling and maturation of the TE constructs. To our knowledge, this is the first cardiovascular tissue-engineering in vivo study using a double-labeling method to demonstrate a direct evidence of the source, persistence, and incorporation into a TE vascular patch of co-cultured and simultaneously pre-seeded adult progenitor cells.

Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector. Publishing Authors By Initials

VL Sales,BA Mettler,M Lopez-Ilasaca,JA Johnson,JE Mayer,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue engineering research

PUBMED ID PMID:

MEDLINE DATE:

Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Tissue engineering

VOLUME: 13

Page Numbers: 525-35

Journal Abbreviation: Tissue Eng.

ISSN: 1076-3279

DAY: 3

MONTH: Mar

YEAR: 2007

Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9505538

Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector. Keywords Mesh Terms:

KEYWORDS: Tissue Engineering

MESH TERMS: genetics

Chemical & Substance for Abstract: Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector. Information

Substance Name: Green Fluorescent Proteins

Registry Number: 147336-22-9

Grant and Affiliation Information for Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector.

AFFILIATION: Department of Cardiovascular Surgery, Children's Hospital, Boston, Massachusetts 02115, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL-68816-01

ACRONYM: HL

MEDLINETA: Tissue Eng

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector Related Publications

• A beta-Hydroxychalcone from Leptospermum scoparium.
• A randomised control trial of structured interrupted generic antiretroviral therapy versus continuous therapy in HIV-infected individuals in Southern India.
• A survey of MSM HIV prevention outreach workers in Chennai, India.
• Alkylcyanoacrylate nanocapsules prepared from mini-emulsions: a comparison with the conventional approach.
• Analysis of fertility indices of cows with extended postpartum anestrus and other reproductive disorders compared to normal cows.
• Analysis of fertility indices of cows with reproductive disorders and of normal cows in herds with low and normal fertility.
• Are we there yet? Distance to care and relative supply among pediatric medical subspecialties.
• Concerted proton-electron transfer in the oxidation of hydrogen-bonded phenols.
• Conformational restriction blocks glutamate receptor desensitization.
• Dual role of 5-HT3 receptors in a rat model of delayed stress-induced visceral hyperalgesia.
• Effect of quercetin feedings on tissue mitochondrial enzymes and performance in mice: 906: june 1 4:00 PM - 4:15 PM.
• Effects of Oat beta-Glucan and Carbohydrate Feedings on Macrophage Antiviral Resistance and Susceptibility to Infection Following Stressful Exercise: 782: May 31 4:30 PM 4:45 PM.
• Endothelial progenitor and mesenchymal stem cell-derived cells persist in tissue-engineered patch in vivo: application of green and red fluorescent protein-expressing retroviral vector.
• Expression of nonclassical class I molecules by intestinal epithelial cells.
• Gene expression profiles of human proximal tubular epithelial cells in proteinuric nephropathies.
• Intestinal epithelial cells from inflammatory bowel disease patients preferentially stimulate CD4+ T cells to proliferate and secrete interferon-gamma.
• Large ground-state entropy changes for hydrogen atom transfer reactions of iron complexes.
• Measuring the stringency of states' indoor tanning regulations: instrument development and outcomes.
• Observations on some environmental factors connected with fertility in heat stressed cows.
• Psychometric properties of the Early Trauma Inventory-Self Report.
• Quercetin Ingestion Does Not Alter Cytokine Changes In Athletes Competing in the Western States Endurance Run: 2476: Board #23 June 2 9:30 AM - 11:00 AM.
• Quercetin reduces illness rates but not immune perturbations following 3 days intensive exercise in cyclists: 784: may 31 5:00 PM 5:15 PM.
• Rectal microbicide acceptability: results of a volume escalation trial.
• Structural characterization of Q10-loaded solid lipid nanoparticles by NMR spectroscopy.
• Support and intervention groups for adolescents with cancer in two Ontario communities.
• Target of rapamycin (TOR) signaling controls epithelial morphogenesis in the vertebrate intestine.
• [In vitro manipulation of primary human osteo-progenitor cells for tissue technological bone production]
• [Networking, an alternative model of community empowerment.]
• [Possibilities for process optimization in orthopedic surgery]
• [The networking model (example and commentary).]