Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression.

Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression. Abstract Text:

Lamis Hammoud,Fuli Xiang,Xiangru Lu,Friedrich Brunner,Kevin Leco,Qingping Feng,

OBJECTIVE: We have recently demonstrated that endothelial nitric oxide synthase (eNOS) promotes cardiomyocyte proliferation. However, mechanisms by which eNOS regulates cardiomyocyte proliferation are not fully understood. The goal of the present study was to investigate the role of tissue inhibitor of metalloproteinase-3 (TIMP-3) in eNOS-mediated cardiomyocyte proliferation. METHODS AND RESULTS: Experiments were conducted in cultured neonatal mouse cardiomyocytes. TIMP-3 expression was significantly decreased in wild-type (WT) cardiomyocytes treated with an adenoviral eNOS (Ad-eNOS). Furthermore, TIMP-3 levels were significantly decreased in cardiomyocytes derived from eNOS transgenic mice. Conversely, TIMP-3 transcript levels were significantly elevated in eNOS(-/-) cardiomyocytes. The inhibitory effect of NO on TIMP-3 expression was dependent on S-nitrosylation of c-jun, a subunit of AP-1. Cell proliferation was increased in TIMP-3(-/-) cardiomyocytes while recombinant TIMP-3 decreased proliferation in both TIMP-3(-/-) and WT cardiomyocytes. Furthermore, the decline in proliferation observed in eNOS(-/-) cardiomyocytes was abrogated when TIMP-3 expression was blocked by an anti-TIMP-3 antibody. In vivo cardiomyocyte proliferation was assessed by Ki67 immunostaining on postnatal day 1 hearts. Ki67-positive cardiomyocytes were decreased in eNOS(-/-), but increased in eNOS-Tg and TIMP-3(-/-) hearts compared to WT controls. CONCLUSIONS: Our study suggests that eNOS promotes neonatal cardiomyocyte proliferation by inhibiting TIMP-3 expression.

Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression. Publishing Authors By Initials

L Hammoud,F Xiang,X Lu,F Brunner,K Leco,Q Feng,

For similar investigative techniques: genetic techniques: gene transfer techniques: transduction, genetic research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transduction, genetic research

PUBMED ID PMID:

MEDLINE DATE:

Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Cardiovascular research

VOLUME: 75

Page Numbers: 359-68

Journal Abbreviation: Cardiovasc. Res.

ISSN: 0008-6363

DAY: 10

MONTH: 05

YEAR: 2007

Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 77427

Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression. Keywords Mesh Terms:

KEYWORDS: Transduction, Genetic

MESH TERMS: methods

Chemical & Substance for Abstract: Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression. Information

Substance Name: Nitric Oxide Synthase Type III

Registry Number: EC 1.14.13.39

Grant and Affiliation Information for Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression.

AFFILIATION: Lawson Health Research Institute, London, Ontario, Canada.

Country: Netherlands

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Cardiovasc Res

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Endothelial nitric oxide synthase promotes neonatal cardiomyocyte proliferation by inhibiting tissue inhibitor of metalloproteinase-3 expression Related Publications

• Effect of the RF cavity temperature on low-energy injection at HLS.
• Preliminary clinical application of contrast-enhanced MR portography.
• Smad6 recruits transcription corepressor CtBP to repress bone morphogenetic protein-induced transcription.
• [Analysis of the Millard method for unilateral cleft lip repair]
• [Assessment of total body water of patients on hemodialysis with urea kinetic model.]
• [Bilateral renal enlargement as the first symptom in a child with acute lymphoblastic leukemia]
• [Comparison of antitumor effects of dendritic cells modified with different forms of hepatocellular cancer antigens]
• [Consequence calculation of a leak for liquid ammonia storage tank]
• [Continuous hemofiltration in the treatment of children's acute renal failure]
• [Determination of amoxicillin and ciprofloxacin hydrochloride soluble powders by RP-HPLC]
• [Determination of organic acids and inorganic anions by gradient ion chromatography]
• [Determination of sexual hormones in cosmetics by using Re-HPLC method]
• [Effect of drying and temperature on the sporulation of Pandora delphacis mycelia gelatinized]
• [Expression activity of maize Ubi-1 promoter in fertile transgenic maize plants]
• [High expression of human thrombopoietin mutant fusion protein in E. coli]
• [Identification of differentially expressed genes in bone marrow CD34+ cells by suppression subtractive hybridization]
• [In vitro reversal of homoharringtonine resistance by the combination of tamoxifen and verapamil]
• [Linkage disequilibrium analysis of -31, -511, -1470 single nucleotide polymorphisms in the promoter of IL-1 beta in Chongqing population]
• [Microcosmic analysis of TCP/HA coating with micro-pores on titanium by IBAD method.]
• [Mutation detection in ED1 gene in hypohidrotic ectodermal dysplasia (HED) families]
• [Preliminary study on oncogene MDM2 in acute leukemias]
• [Progress of phased chest compression cardiopulmonary resuscitation with hands]
• [Rapid diagnosis of common pathogenic bacteria infection in newborn infants by 16SrDNA oligonucleotide array]
• [Studies on the genotoxic effects of acrylonitrile]
• [Study on the immunophenotype of myeloid cells in myelodysplastic syndromes and its clinical implications]
• [The association between gingival cervicular fluid Interleukin-6 level, serum estradiol status and periodontitis in Uyghur postmenopausal women]
• [The expression and significance of peroxisome proliferator-activated receptor-gamma in airway remodeling of asthmatic rats]
• [The expression of apoptosis-associated proteins Bcl-2, Bax in oral leukoplakia and lichen planus]
• [The killing effect of IL-2 and IFN-alpha activated bone marrow on K562 leukemic cell]
• [Variation in hepatitis C virus NS 5b nucleotide sequence and its relationship with interferon treatment]