Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression.

Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression. Research Abstract Details 

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Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression. Abstract Text:

S C Schweitzer,A M Reding,H M Patton,T P Sullivan,C E Stubbs,E Villalobos-Menuey,S A Huber,M K Newell,

Although the immune system, inflammation, and cellular metabolism are linked to diseases associated with dyslipidemias, the mechanism(s) remain unclear. To determine whether there is a mechanistic link between lipid availability and inflammation/immune activation, we evaluated macrophage cell lines incubated under conditions of altered exogenous and endogenous lipid availability. Limiting exogenous lipids results in decreased lysosomal acidity and decreased lysosomal enzymatic activity. Both lysosomal parameters are restored with the addition of oleoyl-CoA, suggesting that fatty acids play a role in the regulation of lysosomal function. Cell surface expression of major histocompatibility complex (MHC)-encoded molecules is also decreased in the absence of exogenous lipids. Additionally, we observe decreased gamma-interferon stimulation of cell surface MHC class II. Using cerulenin to limit the endogenous synthesis of fatty acids results in decreased cell surface expression of MHC class II but does not appear to alter lysosomal acidity, suggesting that lysosomal acidity is dependent on exogenous, but not endogenous, fatty acid availability. Testing these conclusions in an in vivo mouse model, we observed statistically significant, diet-dependent differences in lysosomal acidity and MHC class II cell surface expression. Collectively, these data demonstrate a mechanistic link between lipid availability and early events in the immune response.

Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression. Publishing Authors By Initials

SC Schweitzer,AM Reding,HM Patton,TP Sullivan,CE Stubbs,E Villalobos-Menuey,SA Huber,MK Newell,

For similar animals: animal population groups: animals, inbred strains: mice, inbred strains: mice, inbred c57bl research abstracts see: animals: animal population groups: animals, inbred strains: mice, inbred strains: mice, inbred c57bl research

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Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of lipid research

VOLUME: 47

Page Numbers: 2525-37

Journal Abbreviation: J. Lipid Res.

ISSN: 0022-2275

DAY: 16

MONTH: 08

YEAR: 2006

Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376606

Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression. Keywords Mesh Terms:

KEYWORDS: Mice, Inbred C57BL

MESH TERMS: metabolism

Chemical & Substance for Abstract: Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression. Information

Substance Name: Glucosylceramidase

Registry Number: EC 3.2.1.45

Grant and Affiliation Information for Endogenous versus exogenous fatty acid availability affects lysosomal acidity and MHC class II expression.

AFFILIATION: Colorado University Institute of Bioenergetics, University of Colorado, Colorado Springs, CO, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R01 GM-16562

ACRONYM: GM

MEDLINETA: J Lipid Res

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