Enantioseparation and impurity determination of the enantiomers of novel phenylethanolamine derivatives by high performance liquid chromatography on amylose stationary phase.

Enantioseparation and impurity determination of the enantiomers of novel phenylethanolamine derivatives by high performance liquid chromatography on amylose stationary phase. Research Abstract Details 

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Enantioseparation and impurity determination of the enantiomers of novel phenylethanolamine derivatives by high performance liquid chromatography on amylose stationary phase. Abstract Text:

Jing Yang,Jin Guan,Li Pan,Kun Jiang,Maosheng Cheng,Famei Li,

Simple and efficient analytical HPLC methods using Chiralpak AS-H as chiral stationary phase were developed for direct enantioseparation of 11 novel phenylethanolamine derivatives. The chromatographic experiments were performed in normal phase mode with n-hexane-ethanol-triethylamine (TEA) as mobile phase. Excellent baseline enantioseparation was obtained for most of compounds. The effects of the concentration of organic modifiers and column temperature were studied for the enantiomeric separation. The mechanism of chiral recognition was discussed based on the relationship between the thermodynamic parameters and structures of compounds. It was found that the enantioseparations were all enthalpy driven, and the tert-butyl groups of compounds had significant influence on the chiral recognition. Trantinterol enantiomers were resolved (R(s)=2.73) within 14min using n-hexane-ethanol-TEA (98:2:0.1, v/v/v) as mobile phase with a flow rate of 0.8mLmin(-1) at 30 degrees C. The optimized method was validated for linearity, precision, accuracy and stability in solution and proved to be robust. The limits of detection (LOD) and quantification (LOQ) for (+)-trantinterol were 0.15 and 0.46mugmL(-1). The method was applied for enantiomeric impurity determination of (-)-trantinterol bulk samples.

Enantioseparation and impurity determination of the enantiomers of novel phenylethanolamine derivatives by high performance liquid chromatography on amylose stationary phase. Publishing Authors By Initials

J Yang,J Guan,L Pan,K Jiang,M Cheng,F Li,

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Enantioseparation and impurity determination of the enantiomers of novel phenylethanolamine derivatives by high performance liquid chromatography on amylose stationary phase. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Analytica chimica acta

VOLUME: 610

Page Numbers: 263-7

Journal Abbreviation: Anal. Chim. Acta

ISSN: 1873-4324

DAY: 18

MONTH: 01

YEAR: 2008

Enantioseparation and impurity determination of the enantiomers of novel phenylethanolamine derivatives by high performance liquid chromatography on amylose stationary phase. Information

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LANGUAGE: eng

NlmUniqueID: 370534

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Grant and Affiliation Information for Enantioseparation and impurity determination of the enantiomers of novel phenylethanolamine derivatives by high performance liquid chromatography on amylose stationary phase.

AFFILIATION: Department of Analytical Chemistry, Shenyang Pharmaceutical University, Shenyang 110016, PR China.

Country: Netherlands

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MEDLINETA: Anal Chim Acta

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