Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori.

Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori. Abstract Text:

Nao Yoshizawa,Yoshiharu Takenaka,Hirokazu Yamaguchi,Tsukamoto Tetsuya,Harunari Tanaka,Masae Tatematsu,Sachiyo Nomura,James R Goldenring,Michio Kaminishi,Nao Yoshizawa,Yoshiharu Takenaka,Hirokazu Yamaguchi,Tsukamoto Tetsuya,Harunari Tanaka,Masae Tatematsu,Sachiyo Nomura,James R Goldenring,Michio Kaminishi,

Spasmolytic polypeptide (TFF2)-expressing metaplasia (SPEM) is observed in mucosa adjacent to human gastric cancer and in fundic glands showing oxyntic atrophy in Helicobacter felis-infected mice. Mongolian gerbils infected with Helicobacter pylori (Hp) develop goblet cell intestinal metaplasia and adenocarcinoma, but the presence of SPEM has not been studied in gerbils. We therefore have sought to examine the development of metaplastic mucosal changes in Hp-infected Mongolian gerbils. Mongolian gerbils were assigned to either uninfected controls or infected with Hp at 17 weeks of age. The animals were killed at 17, 20, 26, 31, 41 and 56 weeks of age. Stomach sections were stained using antibodies for TFF2, intrinsic factor, H/K-ATPase, BrdU and MUC2. Dual immunofluorescence staining for TFF2 with intrinsic factor and for TFF2 with MUC2 was performed. In uninfected animals, no SPEM or intestinal metaplasia was observed. Infected gerbils developed SPEM initially in the intermediate zone along the lesser curvature and subsequently spread out towards the greater curvature. In the earlier stages of infection, SPEM glands demonstrated TFF2 and intrinsic factor double staining cells. However, after 35 weeks of infection, the number of double staining SPEM cells decreased. While early in infection SPEM organized in straight glands, in the later stages of infections, SPEM glands became distorted or dilated along with the development of gastritis cystica profunda that was TFF2 positive. Goblet cell intestinal metaplasia developed only late in the infection. Dual staining for TFF2 and MUC2 showed glands containing both SPEM- and MUC2-positive goblet cell intestinal metaplasia. SPEM develops early in Hp infection in Mongolian gerbils, and alterations in gland morphology arise from SPEM glands during the course of gastric infection with goblet cell intestinal metaplasia developing subsequent to SPEM.

Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori. Publishing Authors By Initials

N Yoshizawa,Y Takenaka,H Yamaguchi,T Tetsuya,H Tanaka,M Tatematsu,S Nomura,JR Goldenring,M Kaminishi,N Yoshizawa,Y Takenaka,H Yamaguchi,T Tetsuya,H Tanaka,M Tatematsu,S Nomura,JR Goldenring,M Kaminishi,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Laboratory investigation; a journal of technical m

VOLUME: 87

Page Numbers: 1265-76

Journal Abbreviation: Lab. Invest.

ISSN: 1530-0307

DAY: 16

MONTH: Dec

YEAR: 2007

Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376617

Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori.

AFFILIATION: Department of Gastrointestinal Surgery, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Lab Invest

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori Related Publications

• Distribution of angiogenin and its gene message in colorectal cancer patients and their clinical relevance.
• Emergence of spasmolytic polypeptide-expressing metaplasia in Mongolian gerbils infected with Helicobacter pylori.
• Nutritional comparisons between a pylorus-preserving gastrectomy with and without suprapyloric lymph node clearance: a preliminary report.
• Preoperative evaluation of thyroid pathology in patients with primary hyperparathyroidism.
• Transcriptional changes in Candida albicans Genes by both farnesol and high cell density at an early stage of morphogenesis in N-acetyl-D-glucosamine medium.
• [A three-year survivor case of gastric cancer with peritoneal dissemination-an outpatient with second-line weekly Paclitaxel.]
• [Management of patients with neuroendocrine tumors of the esophagus, stomach, and duodenum]